Rabbit Anti-CCR9 Recombinant Antibody (clone 4G2) (CAT#: ZG-0583J)
This product is a rabbit antibody that recognizes CCR9.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-CCR9 Antibody (ZG-0583J) in WB
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue
All lanes: CCR9 antibody at 1μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 41 KDa
Observed band size: 43 KDa
IF
Figure 2 Rabbit Anti-CCR9 Antibody (ZG-0583J) in IF
Immunofluorescence staining of HepG2 cells with ZG-0583J at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 3 Rabbit Anti-CCR9 Antibody (ZG-0583J) in IP
Immunoprecipitating CCR9 in HL-60 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0583J in HL-60 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0583J (3μg) + HL-60 whole cell lysate (500μg)
Lane 3: HL-60 whole cell lysate (20μg)
Specifications
- Immunogen
- A synthesized peptide derived from human CCR9
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCR9
- Species Reactivity
- Human, Mouse
- Clone
- 4G2
- Applications
- ELISA, WB, IF, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation.
WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000
Target
- Alternative Names
- GPR28; CDw199; GPR-9-6; CC-CKR-9; C-C chemokine receptor type 9; G protein-coupled receptor 28; chemokine (C-C motif) receptor 9
- Gene ID
- 10803
- UniProt ID
- P51686
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-162 | Recombinant Anti-human CCR9 Antibody | WB, Neut, FuncS | IgG |
| MOB-0376CT | Mouse Anti-CCR9 Recombinant Antibody (clone DX-2.3) | FC | Mouse IgG2a |
| HPAB-0039-LS | Mouse Anti-CCR9 Recombinant Antibody (HPAB-0039-LS) | FC, WB, ELISA, FuncS | Mouse IgG |
| HPAB-0040-LS | Mouse Anti-CCR9 Recombinant Antibody (HPAB-0040-LS) | FC, WB, ELISA, Cyt, CDC, FuncS | Mouse IgG |
| VS1-YJ23 | Rat Anti-CCR9 Recombinant Antibody (clone 7E7) | IF, IHC-Fr, FC | Rat IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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