Rabbit Anti-CD247 (pY142) Recombinant Antibody (clone R03-7I5)
CAT#: VS3-WK1997
This product is a rabbit recombinant antibody that is specific to phosphorylated CD247. The target of this antibody is phospho-CD247 (Tyr142). The clone R03-7I5 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of CD247 (pTyr142) observed in WB experiments using this antibody is 19 kDa.
Gene Expression
Normal Tissue
Figure 1 Bone marrow
Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8750/27622_B_5_4.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8750/27622_A_7_8.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198821-CD247
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Tyr142 of human CD3 zeta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr142 of human CD247
- Species Reactivity
- Human
- Clone
- R03-7I5
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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