Rabbit Anti-CD247 (pY142) Recombinant Antibody (clone R03-7I5) (CAT#: VS3-WK1997)
This product is a rabbit recombinant antibody that is specific to phosphorylated CD247. The target of this antibody is phospho-CD247 (Tyr142). The clone R03-7I5 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of CD247 (pTyr142) observed in WB experiments using this antibody is 19 kDa.
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Normal Tissue
RNA Expression
Figure 1 Bone marrow
(Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8750/27622_B_5_4.jpg
(Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8750/27622_B_5_4.jpg
Figure 2 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8750/27622_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8750/27622_A_7_8.jpg
Figure 3 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198821-CD247
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198821-CD247
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Tyr142 of human CD3 zeta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr142 of human CD247
- Species Reactivity
- Human
- Clone
- R03-7I5
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- CD247 Molecule; CD3z Antigen, Zeta Polypeptide (TiT3 Complex); T-Cell Receptor T3 Zeta Chain; CD247 Antigen; CD3Z; TCRZ; T3Z; T-Cell Antigen Receptor Complex, Zeta Subunit Of CD3; T-Cell Surface Glycoprotein CD3 Zeta Chain
- Gene ID
- 919
- UniProt ID
- P20963
- Long Name
- CD247 Molecule
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-171-F(E) | Recombinant Anti-human CD247 Antibody Fab Fragment | WB, IF, FuncS | Fab |
| MHH-171-F(E) | Recombinant Human Anti-human CD247 Antibody Fab Fragment | WB, FC, FuncS | Fab |
| FAMAB-0050-YC-F(E) | Mouse Anti-CD247 Recombinant Antibody Fab Fragment (FAMAB-0050-YC-F(E)) | FC, FuncS | Mouse Fab |
| FAMAB-0051-YC-F(E) | Mouse Anti-CD247 Recombinant Antibody Fab Fragment (FAMAB-0051-YC-F(E)) | FC, FuncS | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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