Human Anti-CD248 Recombinant Antibody (clone 78)
CAT#: VS-0723-CJ8
This human anti-CD248 antibody (clone 78) is a product that recognizes human Endosialin/TEM1. The mAb also cross reacts with mouse Endosialin. This antibody can be used for in vitro assays including flow cytometry analysis and in vivo for targeted-imaging.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1774_B3_8_cr5950f83255423_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1368_F10_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1293_C11_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Goblet cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HSkMC, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000174807-CD248
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human and mouse Endosialin (TEM1)
- Species Reactivity
- Human, Mouse
- Clone
- 78
- Applications
- ELISA, FC, IF
- Conjugate
- Unconjugated
- Related Disease
- Tumors
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody's affinity was tested by ELISA coated with rhTEM1-GST. The binding of this antibody (10 nM) to TEM1-expresser mammalian cells was evaluated using hTEM1-transfected microvascular endothelial cells by flow cytometry.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-289-sdAb | Recombinant Anti-Mouse Cd248 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-773 | Anti-CD248 Recombinant Antibody (Ontuxizumab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2375z | Mouse Anti-CD248 Recombinant Antibody (clone 5B2) | WB, ELISA, IHC | Mouse IgG1 |
| HPAB-AP032-YC | Human Anti-CD248 Recombinant Antibody (HPAB-AP032-YC) | ELISA, FC, WB, FuncS | Human IgG |
| VS7-HM486 | Mouse Anti-CD248 Recombinant Antibody (clone 1H11A10) | FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-114CL | Humanized Anti-Human CD248 Recombinant Antibody | ELISA | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-340MZ | Human Anti-CD248 Recombinant Antibody (TAB-340MZ) | ELISA, FC | Human IgG |
| TAB-340MZ-S(P) | Human Anti-CD248 Recombinant Antibody; scFv Fragment (TAB-340MZ-S(P)) | ELISA, FC | Human scFv |
| TAB-340MZ-F(E) | Human Anti-CD248 Recombinant Antibody; Fab Fragment (TAB-340MZ-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-341MZ | Anti-Human CD248 Recombinant Antibody (FB5) | IHC | |
| TAB-341MZ-S(P) | Anti-Human CD248 Recombinant Antibody scFv Fragment (FB5) | IHC | |
| TAB-341MZ-F(E) | Anti-Human CD248 Recombinant Antibody Fab Fragment (FB5) | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-773 | Afuco™ Anti-CD248 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-773) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0505 | PE-A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer | FCM, IHC | |
| MHC-CN0506 | APC-A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer | FCM, IHC | |
| MHC-CN0507 | FITC-A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer | FCM, IHC | |
| MHC-CN0508 | A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer | FCM, IHC | |
| MHC-YF742 | A*02:01/Human TEM1 (LLVPTCVFLV) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP032-YC-S(P) | Human Anti-CD248 Recombinant Antibody; scFv Fragment (HPAB-AP032-YC-S(P)) | ELISA, FC, WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP032-YC-F(E) | Human Anti-CD248 Recombinant Antibody; Fab Fragment (HPAB-AP032-YC-F(E)) | ELISA, FC, WB, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY55 | Mouse Anti-CD248 (clone FB5) scFv-Fc Chimera | IHC | Mouse IgG1, scFv-Fc |
| VS-0125-FY82 | Human Anti-CD248 (clone 1C1m) scFv-Fc Chimera | FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY29 | CytoStream™ Mouse Anti-CD248 Recombinant Antibody (clone B1/35) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC78 | Recombinant Anti-CD248 Vesicular Antibody, EV Displayed (VS-0425-YC78) | ELISA, FC, Neut, Cell-uptake |
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