Human Anti-CD248 Recombinant Antibody (clone 78) (CAT#: VS-0723-CJ8)
This human anti-CD248 antibody (clone 78) is a product that recognizes human Endosialin/TEM1. The mAb also cross reacts with mouse Endosialin. This antibody can be used for in vitro assays including flow cytometry analysis and in vivo for targeted-imaging.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1774_B3_8_cr5950f83255423_selected.jpg
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1774_B3_8_cr5950f83255423_selected.jpg
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1368_F10_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1368_F10_1_blue_red_green.jpg
Figure 3 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1293_C11_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1293_C11_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_B_9_5.jpg
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_B_9_5.jpg
Figure 5 Colon
(Goblet cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_9_3.jpg
(Goblet cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_8_5.jpg
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HSkMC, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000174807-CD248
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000174807-CD248
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human and mouse Endosialin (TEM1)
- Species Reactivity
- Human, Mouse
- Clone
- 78
- Applications
- ELISA, FC, IF
- Conjugate
- Unconjugated
- Related Disease
- Tumors
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody's affinity was tested by ELISA coated with rhTEM1-GST. The binding of this antibody (10 nM) to TEM1-expresser mammalian cells was evaluated using hTEM1-transfected microvascular endothelial cells by flow cytometry.
Target
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-289-sdAb | Recombinant Anti-Mouse Cd248 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
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