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Human Anti-CD248 Recombinant Antibody (clone 78) (CAT#: VS-0723-CJ8)

This human anti-CD248 antibody (clone 78) is a product that recognizes human Endosialin/TEM1. The mAb also cross reacts with mouse Endosialin. This antibody can be used for in vitro assays including flow cytometry analysis and in vivo for targeted-imaging.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line ASC TERT1
Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1774_B3_8_cr5950f83255423_selected.jpg
Figure 2 IF staining of human cell line BJ
Figure 2 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1368_F10_1_blue_red_green.jpg
Figure 3 IF staining of human cell line Hep G2
Figure 3 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/1293_C11_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_B_9_5.jpg
Figure 5 Colon
Figure 5 Colon
(Goblet cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_9_3.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51856/120429_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HSkMC, U-2197)
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ, BJ hTERT+, fHDF/TERT166, HSkMC, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000174807-CD248

Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human and mouse Endosialin (TEM1)
  • Species Reactivity
  • Human, Mouse
  • Clone
  • 78
  • Applications
  • ELISA, FC, IF
  • Conjugate
  • Unconjugated
  • Related Disease
  • Tumors

Product Property

  • Purification
  • Protein A purified
  • Purity
  • >95%
  • Buffer
  • PBS
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody's affinity was tested by ELISA coated with rhTEM1-GST. The binding of this antibody (10 nM) to TEM1-expresser mammalian cells was evaluated using hTEM1-transfected microvascular endothelial cells by flow cytometry.

Target

  • Alternative Names
  • TEM1; CD164L1; Endosialin; TEM1

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Cd248"

Single-domain Antibody

CAT Product Name Application Type
NAB-289-sdAb Recombinant Anti-Mouse Cd248 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH

Chimeric Antibody

CAT Product Name Application Type
TAB-773 Anti-CD248 Recombinant Antibody (Ontuxizumab) Neut, ELISA, IF, IP, FuncS, FC, ICC IgG1 - kappa

Recombinant Antibody

CAT Product Name Application Type
MOB-2375z Mouse Anti-CD248 Recombinant Antibody (clone 5B2) WB, ELISA, IHC Mouse IgG1
HPAB-AP032-YC Human Anti-CD248 Recombinant Antibody (HPAB-AP032-YC) ELISA, FC, WB, FuncS Human IgG

Humanized Antibody

CAT Product Name Application Type
TAB-114CL Humanized Anti-Human CD248 Recombinant Antibody ELISA Antibody

Human Antibody

CAT Product Name Application Type
TAB-340MZ Human Anti-CD248 Recombinant Antibody (TAB-340MZ) ELISA, FC Human IgG
TAB-340MZ-S(P) Human Anti-CD248 Recombinant Antibody; scFv Fragment (TAB-340MZ-S(P)) ELISA, FC Human scFv
TAB-340MZ-F(E) Human Anti-CD248 Recombinant Antibody; Fab Fragment (TAB-340MZ-F(E)) ELISA, FC Human Fab

Mouse Antibody

CAT Product Name Application Type
TAB-341MZ Anti-Human CD248 Recombinant Antibody (FB5) IHC
TAB-341MZ-S(P) Anti-Human CD248 Recombinant Antibody scFv Fragment (FB5) IHC
TAB-341MZ-F(E) Anti-Human CD248 Recombinant Antibody Fab Fragment (FB5) IHC

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-773 Afuco™ Anti-CD248 ADCC Recombinant Antibody (Ontuxizumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-CN0505 PE-A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer FCM, IHC
MHC-CN0506 APC-A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer FCM, IHC
MHC-CN0507 FITC-A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer FCM, IHC
MHC-CN0508 A*02:01/Human CD248 (LLVPTCVFLV) MHC Tetramer FCM, IHC
MHC-YF742 A*02:01/Human TEM1 (LLVPTCVFLV) MHC Pentamer FCM

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-AP032-YC-S(P) Human Anti-CD248 Recombinant Antibody; scFv Fragment (HPAB-AP032-YC-S(P)) ELISA, FC, WB, FuncS Human scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-AP032-YC-F(E) Human Anti-CD248 Recombinant Antibody; Fab Fragment (HPAB-AP032-YC-F(E)) ELISA, FC, WB, FuncS Human Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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