Rabbit Anti-CD34 Recombinant Antibody (clone R02-5E6) (CAT#: VS3-XY3296)
The rabbit recombinant antibody specifically recognizes CD34 molecule (CD34), which acts as a scaffold for the attachment of lineage specific glycans. It reacts with human, mouse, rat and dog CD34. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36723/1751_G1_7_cr57fd220c70be0_selected.jpg
(Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36723/1751_G1_7_cr57fd220c70be0_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_B_8_5.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_B_8_5.jpg
Figure 3 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_9_3.jpg
Figure 4 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_8_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18/155469_A_8_5.jpg
Figure 5 RNA cell line category: Group enriched (HUVEC TERT2, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174059-CD34
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000174059-CD34
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Dog CD34
- Species Reactivity
- Human, Mouse, Rat, Dog
- Clone
- R02-5E6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Dermatofibrosarcoma Protuberans; Fasciitis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- CD34 molecule
- Gene ID
- 947
- UniProt ID
- P28906
- Long Name
- CD34 molecule
- Cellular Localization
- Membrane
- Post Translation Modifications
- Highly glycosylated. (P28906-CD34_HUMAN)
Phosphorylated on serine residues by PKC. (P28906-CD34_HUMAN)
Glycosylation at Asn36, Asn51, Asn55, Asn85, Asn107, Asn126, Asn142, Asn194, and Asn249 (NX_P28906 [NX_P28906-1])
Modification sites at PhosphoSitePlus (P28906)
- Protein Refseq
- XP_054195678.1
- Function
- Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-175-F(E) | Recombinant Anti-human CD34 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| HPAB-M0472-YC-F(E) | Mouse Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-M0472-YC-F(E)) | WB, IF, IHC, IP, WB | Mouse Fab |
| HPAB-M0473-YC-F(E) | Human Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-M0473-YC-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| HPAB-0883-WJ-F(E) | Mouse Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-0883-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-0884-WJ-F(E) | Human Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-0884-WJ-F(E)) | ELISA | Humanized Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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