Rabbit Anti-CD34 Recombinant Antibody (clone R02-5E6)
CAT#: VS3-XY3296
The rabbit recombinant antibody specifically recognizes CD34 molecule (CD34), which acts as a scaffold for the attachment of lineage specific glycans. It reacts with human, mouse, rat and dog CD34. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36723/1751_G1_7_cr57fd220c70be0_selected.jpg
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Dog CD34
- Species Reactivity
- Human, Mouse, Rat, Dog
- Clone
- R02-5E6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Dermatofibrosarcoma Protuberans; Fasciitis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- CD34 molecule
- Gene ID
- 947
- UniProt ID
- P28906
- Long Name
- CD34 molecule
- Cellular Localization
- Membrane
- Post Translation Modifications
- Highly glycosylated. (P28906-CD34_HUMAN)
Phosphorylated on serine residues by PKC. (P28906-CD34_HUMAN)
Glycosylation at Asn36, Asn51, Asn55, Asn85, Asn107, Asn126, Asn142, Asn194, and Asn249 (NX_P28906 [NX_P28906-1])
Modification sites at PhosphoSitePlus (P28906)
- Protein Refseq
- XP_054195678.1
- Function
- Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-175-F(E) | Recombinant Anti-human CD34 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| MHH-175-F(E) | Recombinant Human Anti-human CD34 Antibody Fab Fragment | Neut, IF, FuncS | Fab |
| HPAB-M0472-YC-F(E) | Mouse Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-M0472-YC-F(E)) | WB, IF, IHC, IP, WB | Mouse Fab |
| HPAB-0883-WJ-F(E) | Mouse Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-0883-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-0884-WJ-F(E) | Human Anti-CD34 Recombinant Antibody; Fab Fragment (HPAB-0884-WJ-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-175-S(P) | Recombinant Anti-human CD34 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| HPAB-M0472-YC-S(P) | Mouse Anti-CD34 Recombinant Antibody; scFv Fragment (HPAB-M0472-YC-S(P)) | WB, IF, IHC, IP, WB | Mouse scFv |
| HPAB-M0473-YC-S(P) | Human Anti-CD34 Recombinant Antibody; scFv Fragment (HPAB-M0473-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-M0474-YC-S(P) | Human Anti-CD34 Recombinant Antibody; scFv Fragment (HPAB-M0474-YC-S(P)) | ELISA | Humanized scFv |
| HPAB-1042-FY-F(E) | Mouse Anti-CD34 Recombinant Antibody (clone c4C8); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-175 | Recombinant Human Anti-human CD34 Antibody | WB, Dot, FuncS | IgG |
| HPAB-1042-FY | Human Anti-CD34 Recombinant Antibody (clone c4C8) | ELISA | Human IgG1, κ |
| HPAB-1043-FY | Human Anti-CD34 Recombinant Antibody (clone C5B12) | FC | Human IgG2a, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-149LC | Anti-Human CD34 Recombinant Antibody (h4C8) | FC, Inhib | Humanized antibody |
| TAB-149LC-S(P) | Anti-Human CD34 Recombinant Antibody scFv Fragment (h4C8) | ELISA | Humanized antibody |
| TAB-149LC-F(E) | Anti-Human CD34 Recombinant Antibody Fab Fragment (h4C8) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-150LC | Human Anti-CD34 Recombinant Antibody (TAB-150LC) | ELISA | Human IgG |
| TAB-150LC-S(P) | Human Anti-CD34 Recombinant Antibody; scFv Fragment (TAB-150LC-S(P)) | ELISA | Human scFv |
| TAB-150LC-F(E) | Human Anti-CD34 Recombinant Antibody; Fab Fragment (TAB-150LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-342CQ | Mouse Anti-CD34 Recombinant Antibody (clone 4H11) | Block, FC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0554 | Hi-Affi™ Rabbit Anti-CD34 Recombinant Antibody (clone DS554AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC90 | AbPlus™ Anti-Cd34 Magnetic Beads (VS-0724-YC90) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0924-YC26 | Rabbit Anti-CD34 Antibody (VS-0924-YC26) - Cancer Stem Cell Marker | IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY92 | Mouse Anti-NHP CD34 Recombinant Antibody (clone 581) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY38 | CytoStream™ Mouse Anti-CD34 Recombinant Antibody (VS-0225-XY38) | FC | Mouse IgG1, kappa |
| VS-0225-XY39 | CytoStream™ Mouse Anti-CD34 Recombinant Antibody (clone 8G12) | FC | Mouse IgG1, kappa |
| VS13-YC161 | CytoStream™ Rabbit Anti-CD34 Polyclonal Antibody (VS13-YC161) | WB, ELISA, IHC-P, IHC-Fr, FC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY362 | Anti-CD34 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1162 | Anti-Rat CD34 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1164 | Anti-Mouse CD34 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1161 | Anti-Human CD34 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC243 | Recombinant Anti-CD34 Vesicular Antibody, EV Displayed (VS-0425-YC243) | ELISA, FC, Inhib, Cell-uptake |
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