Rabbit Anti-CD36 Antibody (VS-0924-YC27) - Cancer Stem Cell Marker
CAT#: VS-0924-YC27
This product is an anti-CD36 antibody (VS-0924-YC27). It targets the human CD36 protein, a marker of cancer stem cells (CSCs). It can be used for detecting CD36 in CSC tissues or cells by Immunohistochemistry at a dilution of 1/50-1/200, and by Immunofluorescence at a concentration of 0.5-2 µg/mL.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2018/1770_D7_3_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2018/7436_A_7_3.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (ASC diff, HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135218-CD36
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD36
- Species Reactivity
- Human
- Applications
- IHC, IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/50-1/200
Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- FAT; GP3B; GP4; GPIV; SCARB3
- Gene ID
- 948
- UniProt ID
- P16671
- Long Name
- CD36 molecule (thrombospondin receptor)
- Cellular Localization
- Cell membrane, Golgi apparatus, Membrane
- Post Translation Modifications
- N-glycosylated and O-glycosylated with a ratio of 2:1.4 Publications
Palmitoylated by ZDHHC5 (PubMed:32958780).
Palmitoylation is required for proper localization at the plasma membrane (PubMed:32958780, PubMed:37461827).
Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:18353783, PubMed:21610069).
Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
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Related Signaling Pathways
Insulin Signaling Pathway
Downloadable Resources
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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