Mouse Anti-CD53 Recombinant Antibody (clone HD77)
CAT#: VS11-QX40
This product is a recombinant mouse antibody that recognizes the extracytoplasmic part of human CD53. This antibody can be used for the following applications: FC, IHC, IF, WB, IP.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_B_7_5.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_7_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_8_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_9_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, HMC-1, Karpas-707, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143119-CD53
❮
❯
Specifications
- Immunogen
- Human leukemia cells
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human CD53
- Species Reactivity
- Human
- Clone
- HD77
- Applications
- FC, IHC, IF, WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- CD53 Molecule; CD53 Antigen; Cell Surface Glycoprotein CD53; Tetraspanin-25; Tspan-25; TSPAN25; MOX44; Antigen MOX44 Identified By Monoclonal Antibody MRC-OX44; Transmembrane Glycoprotein; CD53 Tetraspan Antigen; Cell Surface Antigen; CD53 Glycoprotein
- Gene ID
- 963
- UniProt ID
- P19397
- Long Name
- Leukocyte surface antigen CD53
- Sequence Similarities
- Belongs to the tetraspanin (TM4SF) family.
- Function
- Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CD53"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0569 | Hi-Affi™ Rabbit Anti-CD53 Recombinant Antibody (clone DS569AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ746 | Mouse Anti-CD53 Recombinant Antibody (clone C53-2) | IF, FC | Mouse IgG2a |
| VS3-XY366 | Mouse Anti-CD53 Recombinant Antibody, FITC (clone 4AC53-2) | FC, IF | Mouse IgG2a |
| VS3-XY367 | Mouse Anti-CD53 Recombinant Antibody, PE (clone 4AC53-2) | FC, IF | Mouse IgG2a |
| VS7-HM614 | Mouse Anti-CD53 Recombinant Antibody (clone 1H2C7) | IHC, FCM | Mouse IgG1 |
| VS7-HM615 | Mouse Anti-CD53 Recombinant Antibody (clone 7A8D3) | WB, IHC, FCM | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC542 | AbPlus™ Anti-CD53 Magnetic Beads (VS-0724-YC542) | IP, Protein Purification | |
| VS-0724-YC544 | AbPlus™ Anti-Cd53 Magnetic Beads (VS-0724-YC544) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC686 | Recombinant Anti-CD53 Vesicular Antibody, EV Displayed (VS-0425-YC686) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1199 | Anti-CD53 Immunohistochemistry Kit | IHC |
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