Mouse Anti-CD63 Recombinant Antibody (ZG-0127J)
CAT#: ZG-0127J
This product is a mouse antibody that recognizes CD63.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-CD63 Recombinant Antibody (ZG-0127J) in IHC
Immunohistochemical analysis of paraffin-embedded Malignant melanoma. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-CD63 Recombinant Antibody (ZG-0127J) in IHC
Immunohistochemical analysis of paraffin-embedded Malignant melanoma. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73506/1798_B5_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/ihc_selected.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_4_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26880_B_6_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135404-CD63
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Specifications
- Immunogen
- Synthesized peptide derived from human CD63
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CD63
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
- Alternative Names
- MLA1; ME491; LAMP-3; OMA81H; TSPAN30; CD63 antigen; CD63 antigen (melanoma 1 antigen); granulophysin; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; ocular melanoma-associated antigen; tetraspanin-30; tspan-30
- Gene ID
- 967
- UniProt ID
- P08962
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| HPAB-0071-YJ-S(P) | Human Anti-CD63 Recombinant Antibody (clone Hu33-11A); scFv Fragment | FC, ELISA, IHC | Humanized scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0071-YJ-F(E) | Human Anti-CD63 Recombinant Antibody (clone Hu33-11A); Fab Fragment | FC, ELISA, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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