This product is a rabbit antibody that recognizes CD99.
Figure 1 Rabbit Anti-CD99 Antibody (ZG-0601J) in WB
Western Blot
Positive WB detected in Jurkat whole cell lysate
All lanes CD99 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 KDa
Observed band size: 28 KDa
Figure 2 Rabbit Anti-CD99 Antibody (ZG-0601J) in IHC
IHC image of ZG-0601J diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Rabbit Anti-CD99 Antibody (ZG-0601J) in FC
Overlay histogram showing Jurkat cells stained with ZG-0601J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-400LC | Human Anti-CD99 Recombinant Antibody (TAB-400LC) | FC, ELISA | Chimeric (Mouse/Human) IgG1 |
TAB-400LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-400LC-S(P)) | FC, ELISA | Human scFv |
TAB-400LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-400LC-F(E)) | FC, ELISA | Chimeric (Mouse/Human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-401LC | Human Anti-CD99 Recombinant Antibody (TAB-401LC) | FC, ELISA, ADCC | Human IgG1 |
TAB-401LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-401LC-S(P)) | FC, ELISA | Human scFv |
TAB-401LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-401LC-F(E)) | FC, ELISA | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-402LC | Human Anti-CD99 Recombinant Antibody (TAB-402LC) | ELISA, FC | Human IgG |
TAB-402LC-S(P) | Human Anti-CD99 Recombinant Antibody; scFv Fragment (TAB-402LC-S(P)) | ELISA, FC | Human scFv |
TAB-402LC-F(E) | Human Anti-CD99 Recombinant Antibody; Fab Fragment (TAB-402LC-F(E)) | ELISA, FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0595 | Hi-Affi™ Rabbit Anti-CD99 Recombinant Antibody (clone DS595AB) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-056YJL | Mouse Anti-CD99 Recombinant Antibody (clone CBC062) | WB, FC, FuncS | Mouse IgG3, κ |
ZG-0139J | Mouse Anti-CD99 Recombinant Antibody (ZG-0139J) | IHC-P, IF | Mouse IgG |
ZG-0140J | Mouse Anti-CD99 Recombinant Antibody (ZG-0140J) | IHC-P, IF | Mouse IgG |
VS3-FY327 | Recombinant Mouse Anti-CD99 Antibody (clone 6C7-2G6-2C5) | IHC-P | Mouse IgG |
VS3-FY328 | Recombinant Rabbit Anti-CD99 Antibody (clone R02-4H1) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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