Human Anti-CDH17 Recombinant Antibody (clone D2005)
CAT#: HPAB-2063LY
This product is a chimeric (mouse/human) antibody that recognizes CDH17.
Published Data
Gene Expression
Tested Data
Figure 1 Competition assay of the hybridoma culture supernatant.
All of the data shown are the means-standard deviations of triplicate assays.
Kusano-Arai, O., Iwanari, H., Kudo, S., Kikuchi, C., Yui, A., Akiba, H.,... & Hamakubo, T. (2018). Synergistic cytotoxic effect on gastric cancer cells of an immunotoxin cocktail in which antibodies recognize different epitopes on CDH17. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(1), 1-11.
FC
Figure 2 Specific binding to the cell surface CDH17 was demonstrated by flow cytometry.
The filled histograms indicate anti-CDH17 mAbs, and the open histograms indicate control IgG binding to AGS cells (upper panels) and MKN74 cells (lower panels). The numbers at the top designate the clone number of the anti-CDH17 mAbs. CDH17, Cadherin-17; mAb, monoclonal antibody.
Kusano-Arai, O., Iwanari, H., Kudo, S., Kikuchi, C., Yui, A., Akiba, H.,... & Hamakubo, T. (2018). Synergistic cytotoxic effect on gastric cancer cells of an immunotoxin cocktail in which antibodies recognize different epitopes on CDH17. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(1), 1-11.
ELISA
Figure 3 Dose-dependent binding of the anti-CDH17 mAbs.
All of the data shown are the means-standard deviations of triplicate assay.
Kusano-Arai, O., Iwanari, H., Kudo, S., Kikuchi, C., Yui, A., Akiba, H.,... & Hamakubo, T. (2018). Synergistic cytotoxic effect on gastric cancer cells of an immunotoxin cocktail in which antibodies recognize different epitopes on CDH17. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(1), 1-11.
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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/1030_A11_3_selected.jpg
Normal Tissue
Figure 2 Duodenum
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23614/52777_A_9_2_val_selected.jpg
Normal Tissue
Figure 3 Small intestine
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Paneth cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_6_2.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_5_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, OE19, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079112-CDH17
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SDS-PAGE
Figure 1 Chimeric Anti-CDH17 Monoclonal Antibody (HPAB-2063LY) in SDS-PAGE
SDS-PAGE analysis of HPAB-2063LY in non-reduced (Lane 2) and reduced (Lane 1) conditions.
ELISA
Figure 2 Chimeric Anti-CDH17 Monoclonal Antibody (HPAB-2063LY) in ELISA
ELISA analysis of HPAB-2063LY was performed by coating with Cadherin 17/CDH17 Protein, Human, Recombinant (His Tag).
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
WB
Figure 3 Chimeric Anti-CDH17 Monoclonal Antibody (HPAB-2063LY) in WB
Western blot analysis of HPAB-2063LY was performed with Cadherin 17/CDH17 Protein, Human, Recombinant (His Tag).
HPAB-2063LY incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
DB
Figure 4 Chimeric Anti-CDH17 Monoclonal Antibody (HPAB-2063LY) in DB
Dot Blot analysis of HPAB-2063LY was performed by coating with Cadherin 17/CDH17 Protein, Human, Recombinant (His Tag).
HPAB-2063LY incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
NC: Negative control PC: Positive control
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Specifications
- Host Species
- Human
- Type
- Chimeric (Mouse/Human) IgG
- Specificity
- Human CDH17
- Species Reactivity
- Human
- Clone
- D2005
- Applications
- ELISA
- Conjugate
- None, customized conjugetion is available
Product Property
- Purification
- Protein A/G purified
- Purity
- >95% determined by SDS-PAGE (Reduced conditions), customized purity is available
- Format
- Liquid or lyophilized powder
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- HPT1; CDH16; HPT-1; cadherin-17; HPT-1 cadherin; LI cadherin; cadherin 17, LI cadherin (liver-intestine); cadherin-16; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; intestinal peptide-associated transporter HPT-1; liver-intestine cadherin
- Gene ID
- 1015
- UniProt ID
- Q12864
- Protein Refseq
- NP_001138135.1
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "Clone D2005"
- CAT
- Product Name
See other products for "CDH17"
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|---|---|---|---|
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| BRD-0112MZ | Chicken Anti-CDH17 Polyclonal IgY | WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2061LY-F(E) | Human Anti-CDH17 Recombinant Antibody (clone D2101); Fab Fragment | ELISA | Human Fab |
| HPAB-M0033-YC-F(E) | Mouse Anti-CDH17 Recombinant Antibody; Fab Fragment (HPAB-M0033-YC-F(E)) | ELISA, FC, FuncS, IHC | Mouse Fab |
| HPAB-M0034-YC-F(E) | Mouse Anti-CDH17 Recombinant Antibody; Fab Fragment (HPAB-M0034-YC-F(E)) | ELISA, FC, FuncS, IHC | Mouse Fab |
| HPAB-M0035-YC-F(E) | Human Anti-CDH17 Recombinant Antibody; Fab Fragment (HPAB-M0035-YC-F(E)) | ELISA, FC, FuncS, IHC | Humanized Fab |
| HPAB-M0036-YC-F(E) | Human Anti-CDH17 Recombinant Antibody; Fab Fragment (HPAB-M0036-YC-F(E)) | ELISA, FC, FuncS, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2061LY | Human Anti-CDH17 Recombinant Antibody (clone D2101) | ELISA | Human IgG |
| HPAB-2062LY | Human Anti-CDH17 Recombinant Antibody (clone D2111) | ELISA | Human IgG |
| HPAB-2064LY | Human Anti-CDH17 Recombinant Antibody (clone D2008) | ELISA | Human IgG |
| HPAB-2065LY | Human Anti-CDH17 Recombinant Antibody (clone D2105) | ELISA | Human IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2061LY-S(P) | Human Anti-CDH17 Recombinant Antibody (clone D2101); scFv Fragment | ELISA | Human scFv |
| HPAB-M0033-YC-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (HPAB-M0033-YC-S(P)) | ELISA, FC, FuncS, IHC | Mouse scFv |
| HPAB-M0034-YC-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (HPAB-M0034-YC-S(P)) | ELISA, FC, FuncS, IHC | Mouse scFv |
| HPAB-M0035-YC-S(P) | Human Anti-CDH17 Recombinant Antibody; scFv Fragment (HPAB-M0035-YC-S(P)) | ELISA, FC, FuncS, IHC | Human scFv |
| HPAB-M0036-YC-S(P) | Human Anti-CDH17 Recombinant Antibody; scFv Fragment (HPAB-M0036-YC-S(P)) | ELISA, FC, FuncS, IHC | Human scFv |
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|---|---|---|---|
| VS-0325-FY97 | Human Anti-CDH17 (clone D2101) scFv-Fc Chimera | ELISA, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0325-XY392 | Anti-CDH17 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1290 | Anti-Mouse CDH17 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1289 | Anti-Human CDH17 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0425-YC250 | Recombinant Anti-CDH17 Vesicular Antibody, EV Displayed (VS-0425-YC250) | ELISA, FC, Cell-uptake |
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