Rabbit Anti-CDKN1B Recombinant Antibody (clone 1G2) (CAT#: ZG-0615J)

Figure 1 Rabbit Anti-CDKN1B Antibody (ZG-0615J) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: CDKN1B antibody at 0.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 KDa
Observed band size: 27 KDa

Figure 2 Rabbit Anti-CDKN1B Antibody (ZG-0615J) in IHC

IHC image of ZG-0615J diluted at 1:97.5 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-CDKN1B Antibody (ZG-0615J) in IHC

IHC image of ZG-0615J diluted at 1:97.5 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 4 Rabbit Anti-CDKN1B Antibody (ZG-0615J) in IF

Immunofluorescence staining of HepG2 cells with ZG-0615J at 1:32.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).