Mouse Anti-CEA Recombinant Antibody (clone COL-1) (CAT#: HPAB-0539-WJ)
This product is a mouse antibody specifically binds to carcinoembryonic antigen (CEA).
Specific Inquiry
IHC
Figure 1 Cdon cancer stained with MAI, COL-1.
CEA is expressed primarily on apical membranes and luminal contents. Bar = 75 µm.
Shi, Z. R., Tacha, D., & Itzkowitz, S. H. (1994). Monoclonal antibody COL-1 reacts with restricted epitopes on carcinoembryonic antigen: an immunohistochemical study. Journal of Histochemistry & Cytochemistry, 42(9), 1215-1219.
WB
Figure 2 Western immunoblot with baculovirus rCEA protein using sera from two patients immunized with rV-CEA.
The arrow indicates a band at approximately Mr 120,000 produced by the COL-1 anti-CEA antibody as a positive control. Pre- and postimmunization patient sera were run in duplicate.
Conry, R. M., Allen, K. O., Lee, S. W., Moore, S. E., Shaw, D. R., & LoBuglio, A. F. (2000). Human autoantibodies to carcinoembryonic antigen (CEA) induced by a vaccinia-CEA vaccine. Clinical cancer research, 6(1), 34-41.
ELISA
Figure 3 Binding of polyclonal monkey anti-CEA sera and COL-1 monoclonal anti-CEA antibody to solid-phase human CEA in the presence or absence of soluble human CEA.
Conry, R. M., Allen, K. O., Lee, S. W., Moore, S. E., Shaw, D. R., & LoBuglio, A. F. (2000). Human autoantibodies to carcinoembryonic antigen (CEA) induced by a vaccinia-CEA vaccine. Clinical cancer research, 6(1), 34-41.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CEA
- Species Reactivity
- Human
- Clone
- COL-1
- Applications
- ELISA
- Related Disease
- Cancers
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunohistochemistry, Western Blot and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- Carcinoembryonic antigen; CEA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
scFv Fragment Antibody
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Single-domain Antibody
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IgM Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-037 | Mouse Anti-CEA Recombinant Antibody (clone MFE-23) | ELISA, WB, FuncS | Mouse IgG |
| PABL-038 | Mouse Anti-CEA Recombinant Antibody (clone T84.66) | ELISA, WB, FuncS | Mouse IgG |
| HPAB-0348CQ | Mouse Anti-CEA Recombinant Antibody (clone H310A/H435Q); scFv Fragment | ELISA, WB, FuncS | Mouse scFv |
| FAMAB-0084JF | Mouse Anti-CEA Recombinant Antibody (clone C5) | IHC | Mouse IgG2b, κ |
| FAMAB-0085JF | Mouse Anti-CEA Recombinant Antibody (clone C7) | WB, ELISA, FuncS | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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