Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F)
CAT#: ZG-0420F
This product is a mouse antibody that recognizes the epitope AA 481-531 of the CHEK2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) in WB.
Western blot analysis of cell lysates from various cell types using Chk2 monoclonal antibody.
IHC
Figure 2 Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), liver cancer (B), breast cancer (C) and Gini cancer (D) showed nuclear localization by DAB staining using Chk2 monoclonal antibody.
IF
Figure 3 Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) in IF.
Confocal immunofluorescence analysis of Hela cells using Chk2 monoclonal antibody (green) shows the nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
Normal Tissue
Figure 2 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
❮
❯
Specifications
- Immunogen
- Recombinant fragment of human Chk2 (aa481-531) expressed in E.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- E. coli CHEK2
- Species Reactivity
- E. coli, Human
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000.
Immunofluorescence: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- Checkpoint Kinase 2; CHK2 Checkpoint Homolog; Cds1 Homolog; HuCds1; RAD53; HCds1; CDS1; CHK2; Serine/Threonine-Protein Kinase Chk2; CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint
- Gene ID
- 11200
- UniProt ID
- O96017
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0420F. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CHEK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4034z | Mouse Anti-CHEK2 Recombinant Antibody (clone 30H3) | WB, ELISA, IF, IHC | Mouse IgG2a, κ |
| MOB-0642MZ | Recombinant Mouse Anti-Human CHEK2 Antibody (clone 9G23) | WB | Mouse antibody |
| MRO-0360-CN | Rabbit Anti-CHEK2 Recombinant Antibody (clone CBACN-147) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK2072 | Rabbit Anti-CHEK2 (pT68) Recombinant Antibody (clone R23-2L-9) | WB, IP | Rabbit IgG |
| VS13-YC218 | Rabbit Anti-CHEK2 Recombinant Antibody (VS13-YC218) | WB, ICC, IF, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0122MZ | Chicken Anti-CHEK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0689 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone DS689AB) | DB, IP, WB | Rabbit IgG |
| MOR-4518 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone TH26DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC455 | AbPlus™ Anti-CHEK2 Magnetic Beads (VS-0724-YC455) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY121 | Mouse Anti-NHP CHEK2 Recombinant Antibody (clone 1C12B8) | WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY446 | Anti-CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1430 | Anti-Mouse CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1431 | Anti-Canine CHEK2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IHC, FuncS, Inhib, Cyt
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application:
-3-1.png)
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF
Application: ELISA, FC, WB, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: Block, IP, IF, FC
-4.jpg)
Application: FC
Application: ELISA, WB
Application: ELISA, Neut
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
