Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F)
CAT#: ZG-0420F
This product is a mouse antibody that recognizes the epitope AA 481-531 of the CHEK2.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) in WB.
Western blot analysis of cell lysates from various cell types using Chk2 monoclonal antibody.
IHC
Figure 2 Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), liver cancer (B), breast cancer (C) and Gini cancer (D) showed nuclear localization by DAB staining using Chk2 monoclonal antibody.
IF
Figure 3 Mouse Anti-CHEK2 Recombinant Antibody (ZG-0420F) in IF.
Confocal immunofluorescence analysis of Hela cells using Chk2 monoclonal antibody (green) shows the nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.
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Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
Normal Tissue
Figure 2 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
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Specifications
- Immunogen
- Recombinant fragment of human Chk2 (aa481-531) expressed in E.
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Specificity
- E. coli CHEK2
- Species Reactivity
- E. coli, Human
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000.
Immunofluorescence: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- Checkpoint Kinase 2; CHK2 Checkpoint Homolog; Cds1 Homolog; HuCds1; RAD53; HCds1; CDS1; CHK2; Serine/Threonine-Protein Kinase Chk2; CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint
- Gene ID
- 11200
- UniProt ID
- O96017
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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