Mouse Anti-Citrullinated epitope Recombinant Antibody (clone 12G1) (CAT#: HPAB-AP605-YC)

Figure 1 12G1 detected citrullinated peptides in the arthritic joints of various experimental arthritis models.

Arthritic joints of each arthritis model were immunostained for citrullinated peptides using 12G1. Figures of the lower panel are the magnifications of the highlighted areas of the upper panel.

Kim, Y., Lee, J., Jung, H., Yi, H., Rim, Y. A., Jung, S. M., & Ju, J. H. (2015). Development of synthetic anti‐cyclic citrullinated peptide antibody and its arthritogenic role. Clinical & translational immunology, 4(11), e51.

Figure 2 12G1 identified RA-associated citrullinated proteins in the arthritic joints of experimental arthritis models.

Hind paws of each arthritis model were subject to confocal immunostaining for collagen. 4′,6-Diamidino-2-phenylindole staining was performed to investigate whether the citrullinated proteins were located intracellularly or extracellularly. Protein expression was quantified based on staining intensity.

Kim, Y., Lee, J., Jung, H., Yi, H., Rim, Y. A., Jung, S. M., & Ju, J. H. (2015). Development of synthetic anti‐cyclic citrullinated peptide antibody and its arthritogenic role. Clinical & translational immunology, 4(11), e51.

Figure 3 Generation of the citrulline-specific mAb, 12G1.

Sera from nonimmunized and immunized mice and the supernatant from the hybridoma cells underwent ELISA to detect CCP or CRP. 12G1 specifically detected CCP.

Kim, Y., Lee, J., Jung, H., Yi, H., Rim, Y. A., Jung, S. M., & Ju, J. H. (2015). Development of synthetic anti‐cyclic citrullinated peptide antibody and its arthritogenic role. Clinical & translational immunology, 4(11), e51.

Figure 4 CIA was induced using a single immunization without booster when 12G1 was additionally injected.

Representative histological features of the joints of each arthritis model. The results of hematoxylin and eosin (figures of the lower panel are the magnifications of the highlighted areas of the upper panel), Safranin O and Toluidine Blue staining. Bn, bone; HC, hyaline cartilage; JS, joint space; Pn, inflamed pannus.

Kim, Y., Lee, J., Jung, H., Yi, H., Rim, Y. A., Jung, S. M., & Ju, J. H. (2015). Development of synthetic anti‐cyclic citrullinated peptide antibody and its arthritogenic role. Clinical & translational immunology, 4(11), e51.

Figure 1 Mouse Anti-Citrullinated epitope Recombinant Antibody (HPAB-AP605-YC) in SDS-PAGE

SDS-PAGE analysis of HPAB-AP605-YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-230 kDa.

Figure 2 Mouse Anti-Citrullinated epitope Recombinant Antibody (HPAB-AP605-YC) in HPLC

The purity of HPAB-AP605-YC was greater than 95% as determined by SEC-HPLC.

Figure 3 Mouse Anti-Citrullinated epitope Recombinant Antibody-Biotin (HPAB-AP605-YC) in WB

Lane 1: HPAB-AP605-YC in non-reducing condition (500 ng)
Lane 2: HPAB-AP605-YC-Biotin in non-reducing condition (500 ng)
Detection agent: SA-HRP