Mouse Anti-COL3A1 Recombinant Antibody (clone PT0118)

CAT#: VS3-CJ285

This product is a mouse antibody that recognizes COL3A1.

Gene Expression
Figure 1 IF staining of human cell line BJ Figure 2 IHC staining of human nasopharynx Figure 3 IHC staining of human bronchus Figure 4 IHC staining of human smooth muscle Figure 5 Cerebral cortex Figure 6 Colon Figure 7 Testis Figure 8 RNA cell line category: Cell line enhanced (AF22, ASC diff, ASC TERT1, fHDF/TERT166, HHSteC, HSkMC)

Specifications

  • Immunogen
  • Synthesized peptide derived from human Collagen Type III
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human COL3A1
  • Species Reactivity
  • Human
  • Clone
  • PT0118
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 50% Glycerol, 0.5% BSA, PBS.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500), Immunofluorescence (1:500-1:200).

Target

  • Alternative Names
  • Collagen alpha-1(III) chain; EDS4A; EDSVASC
  • Sequence Similarities
  • Belongs to the fibrillar collagen family.
  • Cellular Localization
  • Extracellular matrix, Secreted
  • Post Translation Modifications
  • Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Function
  • Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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MOB-4057z Mouse Anti-COL3A1 Recombinant Antibody (clone 30D6) WB, IP, IF, ELISA Mouse IgG1, κ
ZG-0640F Rabbit Anti-COL3A1 Recombinant Antibody (ZG-0640F) IHC-P, IF, ELISA, RIA, WB Rabbit IgG
ZG-0642F Rabbit Anti-COL3A1 Recombinant Antibody (ZG-0642F) IHC-P, IF, ELISA, RIA, WB Rabbit IgG
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CAT Product Name Application Type
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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