Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0647F)
CAT#: ZG-0647F
This product is a rabbit antibody that recognizes COL4A1.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human placenta
Immunohistochemical staining of human placenta shows positivity in basement membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/5012_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_4_6.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1695/4979_A_3_7.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, EFO-21, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187498-COL4A1
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Specifications
- Immunogen
- Type IV collagen purified from rodent tumor tissue
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Species Reactivity
- Rat, Mouse
- Applications
- Immunohistochemistry-Paraffin, Immunofluorescence, ELISA, Radioimmunoassay, Western Blot
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Immunofluorescence: 1: 80
Immunohistochemistry-Paraffin: 1: 500
ELISA: 1: 200
Target
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Consistent Performance
I have used the Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0647F) for over a year now in Western Blot analyses. It consistently provides clean, distinct bands with minimal background noise. Highly reliable!
Reliable in IF
We used this antibody for Immunofluorescence studies and were impressed with the strong, specific fluorescent signals. The minimal background made it easy to interpret results.
Good Dilution Range
I appreciated the flexibility in dilution ratios for different applications. It worked effectively in both Western Blot and Immunofluorescence at recommended concentrations.
Q&As
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Has this antibody been tested for Western Blot applications?
A: Yes, the Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0647F) works incredibly well in Western Blot, producing clear, distinct bands with the predicted molecular weight of COL4A1.
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How does this antibody perform in fluorescence assays?
A: It produces strong fluorescence staining in fixed cells and tissue slices with negligible background interference.
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What is the isotype of this antibody?
A: The Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0647F) is an IgG isotype.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "COL4A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2110z | Mouse Anti-COL4A1 Recombinant Antibody (clone 35A2) | WB, IHC | Mouse IgG1 |
| ZG-0178J | Mouse Anti-COL4A1 Recombinant Antibody (clone 4D1) | IHC-P, IF | Mouse IgG |
| ZG-0645F | Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0645F) | IHC-P, IF, ELISA, RIA | Rabbit IgG |
| ZG-0646F | Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0646F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
| ZG-0648F | Rabbit Anti-COL4A1 Recombinant Antibody (ZG-0648F) | IHC-P, IF, ELISA, RIA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC236 | CytoStream™ Rabbit Anti-COL4A1 Polyclonal Antibody (VS13-YC236) | ELISA, IHC-P, IHC-Fr, FC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1590 | Anti-COL4A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1591 | Anti-Mouse COL4A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1592 | Anti-Rat COL4A1 Immunohistochemistry Kit | IHC |
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