Rabbit Anti-CTNND1 (pT916) Recombinant Antibody (clone R07-5A6)
CAT#: VS3-WK2023
This product is a rabbit recombinant antibody that is specific to phosphorylated CTNND1. The target of this antibody is phospho-CTNND1 (Thr916). The clone R07-5A6 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, and IHC staining at a dilution of 1/50-1/100. The molecular weight of CTNND1 (pThr916) observed in WB experiments using this antibody is 100 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15955/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3837/if_selected.jpg
Normal Tissue
Figure 3 Small intestine
High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15954/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15955/36069_A_6_2_selected.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15955/36069_A_7_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15955/36069_A_9_8.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/15955/36069_A_7_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198561-CTNND1
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WB
Figure 1 Rabbit Anti-CTNND1 (pThr916) Recombinant Antibody (clone R07-5A6) in WB.
Western blot analysis of phospho-delta 1 Catenin/CAS (Thr916) in MCF-7, U251, C2C12 lysates using anti-CTNND1 (pThr916) antibody, Cat# VS3-WK2023.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Thr916 of human delta 1 Catenin/CAS
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Thr916 of human and mouse CTNND1
- Species Reactivity
- Human, Mouse
- Clone
- R07-5A6
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "CTNND1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3971z | Mouse Anti-CTNND1 Recombinant Antibody (clone 43H7) | WB, IP, IF | Mouse IgG1, κ |
| MOB-0047CT | Recombinant Mouse anti-Human CTNND1 Monoclonal antibody (3345D7b) | Dot, ICC, WB | |
| VS3-FY2680 | Rabbit Anti-CTNND1 Recombinant Antibody (VS3-FY2680) | WB, IHC, IP | Rabbit IgG |
| VS3-WK1986 | Rabbit Anti-CTNND1 (pY228) Recombinant Antibody (clone R08-5A8) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK2022 | Rabbit Anti-CTNND1 (pS268) Recombinant Antibody (clone R06-0H8) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0862 | Hi-Affi™ Rabbit Anti-CTNND1 Recombinant Antibody (clone DS862AB) | ICC, IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1773 | Anti-CTNND1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1774 | Anti-Mouse CTNND1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1775 | Anti-Rat CTNND1 Immunohistochemistry Kit | IHC |
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