Mouse Anti-CXCL8 Recombinant Antibody (VS3-CJ554)

Name: Mouse Anti-CXCL8 Recombinant Antibody (VS3-CJ554)
SKU: VS3-CJ554
Rating: 5 (1 reviews)

CAT#: VS3-CJ554

This product is a mouse antibody that recognizes human, mouse, and rat CXCL8.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/1010_F11_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non germinal center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line GAMG

Immunofluorescent staining of human cell line GAMG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/2058_E8_1_blue_red_green.jpg

Normal Tissue Figure 4 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_7_5.jpg

Normal Tissue Figure 5 Testis

Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_6_6.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Group enriched (BJ hTERT+, GAMG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169429-CXCL8

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Specifications

Immunogen
Synthetic C-term peptide of IL-8

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human, Mouse, Rat CXCL8

Species Reactivity
Human, Mouse, Rat

Applications
IHC

Conjugate
Unconjugated

Product Property

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
50% Glycerol, 0.5% BSA, PBS.

Preservative
0.02% Sodium Azide

Storage
Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Immunohistochemistry (1:100-1:200).

Target

Alternative Names
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1; SCYB8

Gene ID
3576

UniProt ID
P10145

Sequence Similarities
Belongs to the intercrine alpha (chemokine CxC) family.

Cellular Localization
Secreted

Post Translation Modifications
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.
(Microbial infection) Cleaved by group A Streptococcus protease SpyCEP; leading to impaired neutrophil endothelial transmigration and thus increased virulence.

Protein Refseq
NP_000575.1

Function
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:7636208, PubMed:18692776).
Also plays an important role in neutrophil activation (PubMed:9623510, PubMed:2145175).
Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716).
G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:8662698, PubMed:11971003).

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-CJ554.pdf

MSDS

MSDS - VS3-CJ554.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Secondary Antibody

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Product Name

Recommended Dilution Buffer

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Product Name

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