Mouse Anti-CXCL8 Recombinant Antibody (VS3-CJ554)
CAT#: VS3-CJ554
This product is a mouse antibody that recognizes human, mouse, and rat CXCL8.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/1010_F11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line GAMG
Immunofluorescent staining of human cell line GAMG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/2058_E8_1_blue_red_green.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (BJ hTERT+, GAMG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169429-CXCL8
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Specifications
- Immunogen
- Synthetic C-term peptide of IL-8
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse, Rat CXCL8
- Species Reactivity
- Human, Mouse, Rat
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1; SCYB8
- Gene ID
- 3576
- UniProt ID
- P10145
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.
(Microbial infection) Cleaved by group A Streptococcus protease SpyCEP; leading to impaired neutrophil endothelial transmigration and thus increased virulence.
- Protein Refseq
- NP_000575.1
- Function
- Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:7636208, PubMed:18692776).
Also plays an important role in neutrophil activation (PubMed:9623510, PubMed:2145175).
Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716).
G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:8662698, PubMed:11971003).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-738-sdAb | Recombinant Anti-human CXCL8 VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201CL | Human Anti-CXCL8 Recombinant Antibody (TAB-201CL) | ELISA | Human IgG2 |
| TAB-701LC | Human Anti-CXCL8 Recombinant Antibody (TAB-701LC) | ELISA, FC, FuncS | Human IgG1, κ |
| TAB-701LC-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (TAB-701LC-S(P)) | ELISA | Human scFv |
| TAB-701LC-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (TAB-701LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-095LC | Recombinant Anti-Human CXCL8 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-164LC | Recombinant Anti-Human CXCL8 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-644CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL601) | ELISA, Neut | Mouse IgG1 |
| NEUT-645CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL602) | WB, ICC, Neut | Mouse IgG1 |
| NEUT-646CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL115) | FC, WB, IHC, Neut | Mouse IgG1 |
| NEUT-647CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone I8-60) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-648CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone I8-S2) | ELISA, Neut, WB | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-660CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone B-K8) | Block, ICFC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4128 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone SI199DS) | ELISA | Rabbit IgG |
| MOR-4624 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH137DS) | WB | Rabbit IgG |
| MOR-4625 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH138DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0059-YC-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (HPAB-N0059-YC-S(P)) | FuncS | Human scFv |
| HPAB-1101-FY-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 656.35); scFv Fragment | ELISA, IA, Block | Mouse scFv |
| HPAB-1102-FY-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 197.2); scFv Fragment | ELISA, IA, Block | Mouse scFv |
| HPAB-2094-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone WS-4); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1544WJ-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (clone K2.2.1) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1101-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 656.35) | ELISA, IA, Block | Mouse IgG |
| HPAB-1102-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 197.2) | ELISA, IA, Block | Mouse IgG |
| HPAB-1103-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 81.1) | ELISA, IA, Block | Mouse IgG |
| HPAB-1104-FY | Mouse Anti-CXCL8 Recombinant Antibody (HPAB-1104-FY) | ELISA | Mouse IgG |
| HPAB-1310WJ | Human Anti-CXCL8 Recombinant Antibody (clone M1-10) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1310WJ-F(E) | Human Anti-CXCL8 Recombinant Antibody (clone M1-10); Fab Fragment | ELISA | Human Fab |
| HPAB-1311WJ-F(E) | Human Anti-CXCL8 Recombinant Antibody (clone M2-11); Fab Fragment | ELISA | Human Fab |
| HPAB-1312WJ-F(E) | Human Anti-CXCL8 Recombinant Antibody (clone M1-3); Fab Fragment | ELISA | Human Fab |
| HPAB-1317WJ-F(E) | Recombinant Mouse Anti-CXCL8 Antibody Fab Fragment (5.12.14) | ELISA | Mouse Fab |
| HPAB-1318WJ-F(E) | Recombinant Mouse Anti-CXCL8 Antibody Fab Fragment (6G4.2.5) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY593 | Anti-CXCL8 Immunohistochemistry Kit | IHC |
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