Mouse Anti-DES Recombinant Antibody (VS3-CJ718) (CAT#: VS3-CJ718)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence within human Desmin

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human DES

• Species Reactivity
• Human

• Applications
• IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.2% BSA, PBS.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Immunohistochemistry (1:100-1:300).

• Alternative Names
• CSM1; CSM2; CDCD3; LGMD1D; LGMD1E; LGMD2R

• Gene ID
• 1674

• UniProt ID
• P17661

• Sequence Similarities
• Belongs to the intermediate filament family.

• Cellular Localization
• Cell membrane, Cytoplasm, Intermediate filament, Membrane, Nucleus

• Post Translation Modifications
• ADP-ribosylation prevents ability to form intermediate filaments.
  Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.
  Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation.

• Protein Refseq
• NP_001918.3

• Function
• Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400).
  In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190).
  May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS