SmartAb™ Recombinant Anti-DLK1 pH-dependent Antibody (VS-0825-YC100)
CAT#: VS-0825-YC100
The anti-DLK1 pH-dependent antibody (VS-0825-YC100) is a pH-responsive antibody engineered to target human DLK1. This pH-switched monoclonal antibody (mAb) was developed by incorporating histidine residues into its variable regions, which results in pH-biased binding to DLK1. By leveraging the natural pH variations in different tissues and cellular compartments, this pH-responsive antibody facilitates precise targeting of DLK1 on cells and tissues. Human DLK1, or Delta-like protein 1, is a transmembrane protein that is involved in cell differentiation and development. It is a member of the Notch ligand family and is associated with various developmental disorders and cancers.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62262/1219_G12_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pituitary gland
Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in anterior lobe.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/62262/1270_642_200x200.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5872/167264_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5872/167264_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (AF22, Hep G2, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185559-DLK1
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Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- IgG1 kappa (combining the mouse antibody's antigen-binding variable regions with a human IgG1 constant region)
- Specificity
- Human DLK1
- Species Reactivity
- Human
- Applications
- ELISA, FC, IHC
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-282 | Recombinant Anti-human DLK1 Antibody | WB, Dot, FuncS | IgG |
| MHH-282 | Recombinant Human Anti-human DLK1 Antibody | WB, IF, FuncS | IgG |
| MOB-0200CT | Recombinant Mouse anti-Human DLK1 Monoclonal antibody (NN0625-0E9) | FC, ICC, IF, IHC-P, WB | |
| VS3-XY582 | Mouse Anti-DLK1 Recombinant Antibody (clone 3A10) | ELISA, WB, ICC, FC | Mouse IgG2b |
| VS7-HM823 | Mouse Anti-DLK1 Recombinant Antibody (clone CBL200HM) | WB, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-282-F(E) | Recombinant Anti-human DLK1 Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| MHH-282-F(E) | Recombinant Human Anti-human DLK1 Antibody Fab Fragment | FC, IP, FuncS | Fab |
| HPAB-0789-CN-F(E) | Mouse Anti-DLK1 Recombinant Antibody; Fab Fragment (HPAB-0789-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0790-CN-F(E) | Human Anti-DLK1 Recombinant Antibody; Fab Fragment (HPAB-0790-CN-F(E)) | ELISA, FC, IHC | Chimeric (mouse/human) Fab |
| HPAB-0791-CN-F(E) | Human Anti-DLK1 Recombinant Antibody; Fab Fragment (HPAB-0791-CN-F(E)) | ELISA, FC, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-282-S(P) | Recombinant Anti-human DLK1 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-282-S(P) | Recombinant Human Anti-human DLK1 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| HPAB-0789-CN-S(P) | Mouse Anti-DLK1 Recombinant Antibody; scFv Fragment (HPAB-0789-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0790-CN-S(P) | Mouse Anti-DLK1 Recombinant Antibody; scFv Fragment (HPAB-0790-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0791-CN-S(P) | Human Anti-DLK1 Recombinant Antibody; scFv Fragment (HPAB-0791-CN-S(P)) | ELISA, FC, IHC | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-215CL | Human Anti-DLK1 Recombinant Antibody (TAB-215CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-202MZ | Mouse Anti-DLK1 Recombinant Antibody (TAB-202MZ) | FC, FuncS | Mouse IgG2a |
| TAB-202MZ-F(E) | Mouse Anti-DLK1 Recombinant Antibody; Fab Fragment (TAB-202MZ-F(E)) | FC | Mouse Fab |
| TAB-203MZ-F(E) | Mouse Anti-DLK1 Recombinant Antibody; Fab Fragment (TAB-203MZ-F(E)) | FC | Mouse Fab |
| TAB-204MZ-F(E) | Mouse Anti-DLK1 Recombinant Antibody; Fab Fragment (TAB-204MZ-F(E)) | FC | Mouse Fab |
| TAB-205MZ-F(E) | Rat Anti-DLK1 Recombinant Antibody; Fab Fragment (TAB-205MZ-F(E)) | FC | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-206MZ-F(E) | Anti-Human DLK1 Recombinant Antibody Fab Fragment (B09) | WB, IP, ELISA | Human antibody |
| TAB-207MZ-F(E) | Anti-Human DLK1 Recombinant Antibody Fab Fragment (A04) | WB, IP, ELISA | Human antibody |
| TAB-208MZ-F(E) | Anti-Human DLK1 Recombinant Antibody Fab Fragment (A05) | WB, IP, ELISA | Human antibody |
| TAB-209MZ-F(E) | Anti-Human DLK1 Recombinant Antibody Fab Fragment (A10) | WB, IP, ELISA | Human antibody |
| TAB-210MZ-F(E) | Anti-Human DLK1 Recombinant Antibody Fab Fragment (H06) | WB, IP, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0999 | Hi-Affi™ Rabbit Anti-DLK1 Recombinant Antibody (clone DS999AB) | WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0565 | PE-A*02:01/Human DLK1 (ILGVLTSLV) MHC Tetramer | FCM, IHC | |
| MHC-CN0566 | APC-A*02:01/Human DLK1 (ILGVLTSLV) MHC Tetramer | FCM, IHC | |
| MHC-CN0567 | FITC-A*02:01/Human DLK1 (ILGVLTSLV) MHC Tetramer | FCM, IHC | |
| MHC-CN0568 | A*02:01/Human DLK1 (ILGVLTSLV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY78 | AbPlus™ Anti-DLK1 Magnetic Beads (B09) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY673 | Anti-DLK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2015 | Anti-Mouse DLK1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC213 | Recombinant Anti-DLK1 Vesicular Antibody, EV Displayed (VS-0425-YC213) | ELISA, FC, Cell-uptake |
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