Human Anti-DNA Recombinant Antibody (clone 71F12)
CAT#: PABC-515
This product is a recombinant Human antibody that can recognize DNA. The antibody is capable to bind to both single-stranded (ss) and dsDNA at high affinity.
Published Data
ELISA
Figure 1 Reversion assay in ELISA.
The reactivity of patient-derived mutated (black: SHM [+]) or reverted (red: SHM [-]) antibodies in ELISA for the respective self-antigens are shown.
Sakakibara, S., Arimori, T., Yamashita, K., Jinzai, H., Motooka, D., Nakamura, S., ... & Narazaki, M. (2017). Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus. Scientific reports, 7(1), 16428.
IF
Figure 2 Reversion assay in IFA.
Sakakibara, S., Arimori, T., Yamashita, K., Jinzai, H., Motooka, D., Nakamura, S., ... & Narazaki, M. (2017). Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus. Scientific reports, 7(1), 16428.
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Specifications
- Host Species
- Human
- Type
- Human IgG2, λ
- Clone
- 71F12
- Applications
- ELISA, IF
- Related Disease
- Systemic lupus erythematosus (SLE)
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay and Immunofluorescence. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- DNA; deoxyribonucleic acid
REVIEWS AND Q&AS
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Complete Procedures Available
The offered protocols ensured that the product was successfully implemented in my tests because they were clear and easy to follow. It streamlined and expedited my work.
Simple Data Visualization
This tool produces a comprehensible and easily interpreted data visualization. It provides high-quality results that are easy to analyze.
Simple to Manage
This antibody was easy to work with and straightforward. It is a dependable reagent because the performance was consistently high and the directions were clear.
Q&As
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What secondary detection methods can I use with this antibody?
A: Depending on your test requirements, you can use a variety of secondary detection methods such as enzymatic (HRP, AP), fluorescent dyes, or chemiluminescence.
-
Do I need to include a blocking step in my protocol?
A: Yes, blocking is critical for reducing non-specific binding. Common blockers include BSA, milk, or serum derived from the secondary antibody's host species.
-
Which buffers should I use to dilute this antibody?
A: PBS or TBS with a tiny amount of BSA or skim milk (0.1-1%) is commonly used to dilute the antibody.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 71F12"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| FAMAB-0075CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 16-13); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0076CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 1A11); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0077CQ-F(E) | Mouse Anti-DNA Recombinant Antibody Fab Fragment (FAMAB-0077CQ-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| PABX-048-S (P) | Recombinant Mouse Anti-DNA Antibody scFv Fragment | WB, ELISA, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0075CQ | Mouse Anti-DNA Recombinant Antibody (clone 16-13) | ELISA | Mouse IgG2a |
| FAMAB-0076CQ | Mouse Anti-DNA Recombinant Antibody (clone 1A11) | ELISA | Mouse IgG2b |
| FAMAB-0077CQ | Mouse Anti-DNA Recombinant Antibody (FAMAB-0077CQ) | ELISA | Mouse IgG2a |
| FAMAB-0078CQ | Mouse Anti-DNA Recombinant Antibody (clone 2F2) | ELISA | Mouse IgG2b |
| FAMAB-0079CQ | Mouse Anti-DNA Recombinant Antibody (FAMAB-0079CQ) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0075CQ-S(P) | Mouse Anti-DNA Recombinant Antibody (clone 16-13); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0076CQ-S(P) | Mouse Anti-DNA Recombinant Antibody (clone 1A11); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0077CQ-S(P) | Mouse Anti-DNA Recombinant Antibody scFv Fragment (FAMAB-0077CQ-S(P)) | ELISA | Mouse scFv |
| FAMAB-0078CQ-S(P) | Mouse Anti-DNA Recombinant Antibody (clone 2F2); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0079CQ-S(P) | Mouse Anti-DNA Recombinant Antibody scFv Fragment (FAMAB-0079CQ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-A1364-YJ | Mouse Anti-DNA Recombinant Antibody (clone ET844.3.1) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1124-YJ33 | Mouse Anti-DNA Recombinant Autoantibody (clone G5-8) | ELISA | Mouse lgG2b lambda |
| VS-1124-YJ34 | Mouse Anti-DNA Recombinant Autoantibody (clone G4-20) | ELISA | Mouse IgG2a kappa |
| VS-1124-YJ35 | Mouse Anti-DNA Recombinant Autoantibody (clone G3-47) | ELISA | Mouse IgG2a kappa |
| VS-1124-YJ37 | Mouse Anti-DNA Recombinant Autoantibody (clone G1-5) | ELISA | Mouse IgG2a kappa |
| VS-1124-YJ39 | Mouse Anti-DNA Recombinant Autoantibody (clone G6-23) | ELISA | Mouse lgG2b lambda |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY165 | Human Anti-DNA (clone 71F12) scFv-Fc Chimera | ELISA, IF | Human IgG1, scFv-Fc |
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