Recombinant Mouse Anti-DNA Antibody (ED10)
CAT#: PABW-032
Recombinant Mouse Antibody (ED10) is capable of binding to DNA, expressed in Chinese Hamster Ovary cells (CHO). This antibody can recognize the 5′-end of the 18-mer site 35-18 strand B E2 binding site, in the context of single or doublestranded DNA, with higher affinity for single than for the double-stranded DNA.
Published Data
Figure 1 Northern and Western blot analyses of chDab1 expression.
(A) Northern blots were prepared using poly(A) þ RNA (2 mg/ lane) extracted from the retina (ED5, ED10, ED16), brain (ED5, ED10, ED16), heart (ED5, ED15), liver (ED5, ED10, ED16), kidney (ED7, ED16) and gut (ED16). The filter was sequentially hybridized with 32P-labeled: (i) 532 bp chDab1 cDNA and (ii) actin cDNA. The extra bands obtained with the actin probe in the heart and gut represent tissue-specific actin mRNAs. We consistently find actin RNA to be low in the liver, especially at later developmental stages.
(B) Western blots were prepared using ED4, ED7 and ED16 total chick retina extracts (50 mg protein/lane). The filter was sequentially incubated with rabbit anti-Dab1 antibody (1:5000) and goat anti-actin antibody (1:500). Molecular mass standards (in kDa) areM indicated on the left.
Katyal, S., & Godbout, R. (2004). Alternative splicing modulates Disabled‐1 (Dab1) function in the developing chick retina. The EMBO journal, 23(8), 1878-1888.
Figure 2 RT–PCR analysis of chDab1 deletion and insertion regions.
(A) cDNAs synthesized from poly(A) þ RNA from the retina (ED5, ED7, ED10, ED16) and brain (ED3.5, ED5, ED16) were amplified using P1 and P2 primers for deletion analysis and P3 and P4 primers for insertion analysis. Sizes of amplified bands are indicated. (B) Banding patterns obtained by RT–PCR analysis of ED5, ED10 and ED16 retina using P1 and P4 primers. The lower band is 382 bp and the higher band is 430 bp.
Katyal, S., & Godbout, R. (2004). Alternative splicing modulates Disabled‐1 (Dab1) function in the developing chick retina. The EMBO journal, 23(8), 1878-1888.
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Specifications
- Immunogen
- Deoxyribonucleic acid
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against synthetic DNA
- Clone
- ED10
- Applications
- WB, ELISA
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The DNA antibody has been reported in applications of WB, RT–PCR.
RT–PCR: 1 mg poly(A) þ RNA from the retina ED10 was reverse transcribed using an oligo d(T) primer and Superscript reverse transcriptase (Stratagene). PCR was carried out using 1/20 of the cDNA generated. Primer set P1 and P2 was used for the analysis of the deletion region, primer set P3 and P4 was used for the analysis of the insertion region, and primer set P1 and P4 was used for the analysis of both the deletion and insertion regions.
Target
- Alternative Names
- DNA; Deoxyribonucleic acid; polynucleotides; double heliX
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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