Human Anti-DSG1 Recombinant Antibody (clone (D31)12a/5); Fab Fragment
CAT#: FAMAB-0207-CN-F(E)
This is a human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The clone (D31)12a/5 is a nonpathogenic antibody recognizes both Dsg1 and Dsg3, wherewa it demonstrates only 5–26% inhibition of binding to Dsg3 by EDTA.
Gene Expression
Normal Tissue
Figure 1 Skin
Cells in basal layer
Staining:Medium
Intensity: Moderate
Quantity:>75%
Cells in corneal layer
Staining:High
Intensity: Strong
Quantity: 75%-25%
Cells in granular layer
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining:High
Intensity: Strong
Quantity:>75%
Langerhans cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22128/169913_B_8_1.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134760-DSG1
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human Fab
- Specificity
- Human DSG1
- Species Reactivity
- Human, Mouse
- Clone
- (D31)12a/5
- Applications
- ELISA, IF, IB
- Related Disease
- Pemphigus
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-786-sdAb | Recombinant Anti-Human DSG1 VHH Single Domain Antibody | IP, ChiP, IHC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1095z | Mouse Anti-DSG1 Recombinant Antibody (clone 7A12) | WB, IP, IF, IHC | Mouse IgG1, κ |
| MOB-1095MZ | Recombinant Mouse Anti-Human DSG1 Antibody (clone 38C3) | ICC, IF, IP WB | Mouse antibody |
| FAMAB-0207-CN | Human Anti-DSG1 Recombinant Antibody (clone (D31)12a/5) | ELISA, IF, IB | Human IgG |
| MRO-0497-CN | Rabbit Anti-DSG1 Recombinant Antibody (clone JB19-46) | WB, IHC | Rabbit IgG |
| VS3-XY3338 | Rabbit Anti-DSG1 Recombinant Antibody (clone R07-2K4) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0319CL | Human Anti-DSG1 Recombinant Antibody (TAB-0319CL) | ELISA, IF, IP | Human IgG |
| TAB-0319CL-S(P) | Human Anti-DSG1 Recombinant Antibody; scFv Fragment (TAB-0319CL-S(P)) | ELISA, IF, IP | Human scFv |
| TAB-0319CL-F(E) | Human Anti-DSG1 Recombinant Antibody; Fab Fragment (TAB-0319CL-F(E)) | ELISA, IF, IP | Human Fab |
| TAB-257MZ | Human Anti-DSG1 Recombinant Antibody (TAB-257MZ) | ELISA, Inhib | Human IgG |
| TAB-257MZ-F(E) | Human Anti-DSG1 Recombinant Antibody; Fab Fragment (TAB-257MZ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1053 | Hi-Affi™ Rabbit Anti-DSG1 Recombinant Antibody (clone DS1053AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0206-CN-S(P) | Human Anti-DSG1 Recombinant Antibody (clone (D31)2/28); scFv Fragment | ELISA, IF, IB | Human scFv |
| FAMAB-0207-CN-S(P) | Human Anti-DSG1 Recombinant Antibody (clone (D31)12a/5); scFv Fragment | ELISA, IF, IB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0206-CN-F(E) | Human Anti-DSG1 Recombinant Antibody (clone (D31)2/28); Fab Fragment | ELISA, IF, IB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY698 | Anti-DSG1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2093 | Anti-Human DSG1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC194 | Recombinant Anti-DSG1 Vesicular Antibody, EV Displayed (VS-0425-YC194) | ELISA, FC, Inhib, Cell-uptake |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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