Anti-EPHA3 Antibody Prodrug, Protease Activated (VS-1025-YC66)
CAT#: VS-1025-YC66
The Anti-EPHA3 Antibody Prodrug, Protease Activated (VS-1025-YC66) is an antibody prodrug comprised of a recombinant human antibody targeting human EPHA3, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to EPHA3 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to EPHA3 effectively. EPHA3 (Ephrin Receptor A3) is a receptor tyrosine kinase that, depending on the cancer type, can function as an oncogene or a tumor suppressor. It is often overexpressed in glioblastoma and lung cancer, where its signaling promotes proliferation and invasion. However, its role as a potential target is complicated by its diverse signaling roles.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69390/1819_G10_18_cr59f32f9e0f828_selected.jpg
Normal Tissue
Figure 2 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_B_9_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10462/24404_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (AF22)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044524-EPHA3
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human EPHA3
- Target
- EPHA3
- Species Reactivity
- Human
- Isotype
- IgG, kappa
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.
- Alternative Names
- EK4; ETK; HEK; ETK1; HEK4; TYRO4
- Gene ID
- 2042
- UniProt ID
- P29320
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Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2640z | Mouse Anti-EPHA3 Recombinant Antibody (clone 18F1) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-101LC | Recombinant Anti-Human EPHA3 Antibody (Fc glycosylation/Low fucosylated) | ELISA | Human antibody |
| Gly-101LC-1 | Recombinant Anti-Human EPHA3 Antibody (Fc glycosylation/Low fucosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0651CT | Recombinant Mouse anti-Human EPHA3 Monoclonal antibody (EML056) | ICC, IF, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1165 | Hi-Affi™ Rabbit Anti-EPHA3 Recombinant Antibody (clone DS1165AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-024ML | Anti-Human EPHA3 Recombinant Antibody (TAB-024ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0007-LS | Human Anti-EPHA3 Recombinant Antibody (HPAB-0007-LS) | ELISA, FC | Human IgG κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0007-LS-F(E) | Human Anti-EPHA3 Recombinant Antibody; Fab Fragment (HPAB-0007-LS-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0007-LS-S(P) | Human Anti-EPHA3 Recombinant Antibody; scFv Fragment (HPAB-0007-LS-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-024ML | Afuco™ Anti-EPHA3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-024ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-430CQ | Afuco™ Anti-EPHA3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-430CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0294J | Mouse Anti-EPHA3 Recombinant Antibody (ZG-0294J) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY630 | Mouse Anti-EPHA3 Recombinant Antibody (clone 6C1B6) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY104 | Human Anti-EPHA3 scFv-Fc Chimera | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC154 | Recombinant Anti-EPHA3 Vesicular Antibody, EV Displayed (VS-0425-YC154) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC115 | SmartAb™ Recombinant Anti-EPHA3 pH-dependent Antibody (VS-0825-YC115) | ELISA, IHC, FC, IP, IF | Human IgG1 kappa |
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