Mouse Anti-EPHB4 Recombinant Antibody (ZG-0305J)
CAT#: ZG-0305J
This product is a mouse antibody that recognizes EPHB4.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-EPHB4 Recombinant Antibody (ZG-0305J) in WB
Western Blot analysis using EphB4 Monoclonal Antibody against truncated EphB4 recombinant protein.
IHC
Figure 2 Mouse Anti-EPHB4 Recombinant Antibody (ZG-0305J) in IHC
Immunohistochemistry analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization with DAB staining using Ep
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Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_4_6.jpg
Normal Tissue
Figure 7 Placenta
Decidual cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13537/29984_A_1_7.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196411-EPHB4
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Specifications
- Immunogen
- Purified recombinant fragment of EphB4 expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EPHB4
- Species Reactivity
- Human
- Applications
- WB, IHC, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- HTK; MYK1; HFASD; CMAVM2; LMPHM7; TYRO11; ephrin type-B receptor 4; ephrin receptor EphB4; hepatoma transmembrane kinase; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK
- Gene ID
- 2050
- UniProt ID
- P54760
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2116z | Mouse Anti-EPHB4 Recombinant Antibody (clone 25G10) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| HPAB-1128-FY | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265) | ELISA | Mouse IgG |
| VS3-XY636 | Mouse Anti-EPHB4 Recombinant Antibody (clone 5B5) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS3-XY637 | Mouse Anti-EPHB4 Recombinant Antibody (clone 5B8F7) | ELISA, WB, IHC, ICC | Mouse IgG2a |
| VS3-XY638 | Mouse Anti-EPHB4 Recombinant Antibody (clone 7H4A6) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-001WM | Anti-Human EPHB4 Recombinant Antibody (MOR03640) | ELISA, FC | Human Antibody |
| TAB-002WM | Anti-Human EPHB4 Recombinant Antibody (TAB-002WM) | ELISA | Human Antibody |
| TAB-001WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (MOR03640) | ELISA, FC | Human Antibody |
| TAB-002WM-S(P) | Anti-Human EPHB4 Recombinant Antibody scFv Fragment (TAB-002WM-S(P)) | ELISA | Human Antibody |
| TAB-001WM-F(E) | Anti-Human EPHB4 Recombinant Antibody Fab Fragment (MOR03640) | ELISA, FC | Human Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-003WM | Mouse Anti-EPHB4 Recombinant Antibody (TAB-003WM) | IHC, WB, FC | Mouse IgG2b |
| TAB-003WM-S(P) | Mouse Anti-EPHB4 Recombinant Antibody; scFv Fragment (TAB-003WM-S(P)) | IHC, WB | Mouse scFv |
| TAB-003WM-F(E) | Mouse Anti-EPHB4 Recombinant Antibody; Fab Fragment (TAB-003WM-F(E)) | IHC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1169 | Hi-Affi™ Rabbit Anti-EPHB4 Recombinant Antibody (clone DS1169AB), FITC | FC | Rabbit IgG |
| MOR-4157 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI232DS) | FC | Rabbit IgG |
| MOR-4158 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI233DS) | ELISA, FC | Rabbit IgG |
| MOR-4159 | Hi-Affi™ Rabbit Anti-EphB4 Recombinant Antibody (clone SI234DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0185-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0186-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-0187-CN-S(P) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); scFv Fragment | ELISA, WB, IP | Humanized scFv |
| HPAB-1128-FY-F(E) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0184-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 22E6); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0185-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 30C2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0186-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 1E10); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-0187-CN-F(E) | Human Anti-EPHB4 Recombinant Antibody (clone 4F2); Fab Fragment | ELISA, WB, IP | Humanized Fab |
| HPAB-1128-FY-S(P) | Mouse Anti-EPHB4 Recombinant Antibody (clone MAB265); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY89 | AbPlus™ Anti-EPHB4 Magnetic Beads (22E6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY774 | Anti-EPHB4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2318 | Anti-Mouse EPHB4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2317 | Anti-Human EPHB4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC372 | Recombinant Anti-EPHB4 Vesicular Antibody, EV Displayed (VS-0425-YC372) | ELISA, FC, Cell-uptake |
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