Recombinant Mouse Anti-fHbp Antibody (JAR 3) (CAT#: FAMAB-0140CQ)

Figure 1 Binding of anti-fHbp MAbs

IgG bound to immobilized fHbp was detected with an anti-human kappa light-chain-specific alkaline phosphatase-conjugated antibody. Error bars represent the range in OD values observed in two independent experiments.

Giuntini, S., Reason, D. C., & Granoff, D. M. (2011). Complement-mediated bactericidal activity of anti-factor H binding protein monoclonal antibodies against the meningococcus relies upon blocking factor H binding. Infection and immunity, 79(9), 3751-3759.

Figure 2 Binding of anti-fHbp MAbs (4 μg/ml) with live bacterial cells of N. meningitidis group B strain H44/76.

JAR 3, black dashed line; JAR 5, gray line; MAb502, black line. An irrelevant human MAb (100 μg/ml) served as a negative control (gray filled histogram). The binding curves of the three anti-fHbp MAbs are superimposed.

Giuntini, S., Reason, D. C., & Granoff, D. M. (2011). Complement-mediated bactericidal activity of anti-factor H binding protein monoclonal antibodies against the meningococcus relies upon blocking factor H binding. Infection and immunity, 79(9), 3751-3759.

Figure 3 Factor H–binding protein (fHbp) variant (v.) 1 in outer membrane vesicle (OMV) preparations, as detected by Western blot analysis.

WT, native OMV prepared from the H44/76 wild-type strain; fHbp KO, OMV from a mutant of H44/76 in which the gene encoding fHbp was inactivated; LpxL1 KO OE fHbp, OMV from a mutant of H44/76 with inactivated LpxL1 and overexpressed fHbp; rfHbp, recombinant v.1 protein). The primary antibody was the anti-rfHbp monoclonal antibody JAR 3.

Koeberling, O., Seubert, A., & Granoff, D. M. (2008). Bactericidal antibody responses elicited by a meningococcal outer membrane vesicle vaccine with overexpressed factor H–binding protein and genetically attenuated endotoxin. The Journal of infectious diseases, 198(2), 262-270.

Figure 4 Serum antibody responses to factor H–binding protein (fHbp) (variant 1), NadA, GNA2132, and lipooligosaccharide (LOS) (LpxL1 knockout mutant), as measured by ELISA.

Shown are results for (left to right) mice immunized with aluminum hydroxide (black bars), Norwegian outer membrane vesicle (OMV) vaccine (OMV, Norway) (checkered bars), recombinant protein vaccine (striped bars), and native OMV prepared from H44/76 with inactivated LpxL1 and increased expression of fHbp (OMV, mutant) (gray bars). Error bars show the 95% confidence intervals (CIs) for the geometric mean titers (GMTs).

Koeberling, O., Seubert, A., & Granoff, D. M. (2008). Bactericidal antibody responses elicited by a meningococcal outer membrane vesicle vaccine with overexpressed factor H–binding protein and genetically attenuated endotoxin. The Journal of infectious diseases, 198(2), 262-270.

Figure 5 Effect of temperature on measurement of titers of antibody to lipooligosaccharide (LOS) and factor H–binding protein (fHbp) (variant 1) by ELISA in serum samples from mice immunized with mutant outer membrane vesicle.

Serum samples were incubated for 2 h at 37°C or for 18 h at 4°C. After the plates were washed, bound immunoglobulin was measured by use of goat anti–mouse IgG/A/M conjugated to alkaline phosphatase. Error bars show 95% confidence intervals (CIs) for the geometric mean titers (GMTs).

Koeberling, O., Seubert, A., & Granoff, D. M. (2008). Bactericidal antibody responses elicited by a meningococcal outer membrane vesicle vaccine with overexpressed factor H–binding protein and genetically attenuated endotoxin. The Journal of infectious diseases, 198(2), 262-270.