Rabbit Anti-FOXO1 Antibody (VS-0924-YC39) - Cancer Stem Cell Marker
CAT#: VS-0924-YC39
This product is an anti-FOXO1 antibody (VS-0924-YC39). It targets the human and mouse FOXO1 protein, a marker for cancer stem cells (CSCs). It's suitable for FOXO1 detection in CSC tissues or cells by immunohistochemistry (dilution 1/20-1/50) and Western blot (0.1-0.5 µg/mL).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1252/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/58168_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/58168_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/58168_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/58168_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/58168_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22326/58168_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff, Daudi, HSkMC, U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000150907-FOXO1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse FOXO1
- Species Reactivity
- Human, Mouse
- Applications
- IHC, WB
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/20-1/50
Western Blot (WB): 0.1-0.5 µg/mL
Target
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Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
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Related Signaling Pathways
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "FOXO1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1343 | Hi-Affi™ Rabbit Anti-FOXO1 Recombinant Antibody (clone DS1343AB) | IHC-P, WB, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0601-CN | Rabbit Anti-FOXO1 Recombinant Antibody (clone CBACN-229) | WB, IF, IHC | Rabbit IgG |
| ZG-0358J | Mouse Anti-FOXO1 Recombinant Antibody (ZG-0358J) | WB | Mouse IgG |
| ZG-0359J | Mouse Anti-FOXO1 Recombinant Antibody (ZG-0359J) | WB, IF | Mouse IgG |
| VS3-QX441 | Mouse Anti-FOXO1 Recombinant Antibody (clone 3B6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-WK182 | Mouse Anti-FOXO1 Recombinant Antibody (VS4-WK182) | ELISA, WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY206 | Rabbit Anti-NHP FOXO1 Recombinant Antibody (VS-1024-XY206) | WB, IHC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY858 | Anti-FOXO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2632 | Anti-Mouse FOXO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2631 | Anti-Human FOXO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2633 | Anti-Zebrafish FOXO1 Immunohistochemistry Kit | IHC |
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