Mouse Anti-GFAP Recombinant Antibody (clone 1C91F1)
CAT#: ZG-0231U
Mouse anti-GFAP antibody (clone 1C91F1) displayed high specificity against the GFAP protein of Human, Mouse and Rat.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-GFAP Antibody (ZG-0231U) in WB
Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: GFAP antibody at 2.7µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa
IHC
Figure 2 Mouse Anti-GFAP Antibody (ZG-0231U) in IHC
Immunohistochemistry of paraffin-embedded human glioma using ZG-0231U at dilution of 1:100
IF
Figure 3 Mouse Anti-GFAP Antibody (ZG-0231U) in IF
Immunofluorescent analysis of U251 cells using ZG-0231U at a dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-GFAP Antibody (ZG-0231U) in FC
Overlay histogram showing SH-SY5Y cells stained with ZG-0231U (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1475_F11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/1326_A1_4_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56030/135460_B_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131095-GFAP
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Specifications
- Immunogen
- Recombinant Human GFAP protein (292-432AA)
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human GFAP
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 1C91F1
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Alexander Disease and Central Neurocytoma
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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