Mouse Anti-GFAP Recombinant Antibody (clone 1C91F1) (CAT#: ZG-0231U)

Figure 1 Mouse Anti-GFAP Antibody (ZG-0231U) in WB

Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: GFAP antibody at 2.7µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa

Figure 2 Mouse Anti-GFAP Antibody (ZG-0231U) in IHC

Immunohistochemistry of paraffin-embedded human glioma using ZG-0231U at dilution of 1:100

Figure 3 Mouse Anti-GFAP Antibody (ZG-0231U) in IF

Immunofluorescent analysis of U251 cells using ZG-0231U at a dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 4 Mouse Anti-GFAP Antibody (ZG-0231U) in FC

Overlay histogram showing SH-SY5Y cells stained with ZG-0231U (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.