Human Anti-GPA33 Recombinant Antibody (clone A2) (CAT#: FAMAB-0161WJ)
This product is a human monoclonal antibody derived from phage display library specifically for human A33 transmembrane glycoprotein. This antibody can be used in applications, such as ELISA, WB, FC, IF.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Figure 1 Binding affinity of the anti-A33 antibodies on CT26A33.C3
Functional binding affinity (fKD) of the antibodies A2, K, K.hu,MG,and negative control TA99 (-), measured by flow cytometry on CT26A33.C3 cells.
SPOT
Figure 2 Binding epitopes of the anti-A33 antibodies A2 on the PepSpot membrane (as black spots).
We used SPOT technology and immunodetection of antibody binding events to 15 amino acid-long peptides on a cellulose membrane, spanning the antigen sequence.
IF
Figure 3 Immunofluorescence staining of A33 on human colon and human adenocarcinoma sections.
Detection of A33 on human colon tissue sections and on human colon adenocarcinoma sections with the antibodies A2, K, and MG (green, Alexa Fluor 488).
In vitro
Figure 4 The antibody A2 induces ADCC in vitro.
In vitro ADCC killing of C51A33.A5 and CT26A33.C3 murine adenocarcinoma cells induced by the antibody A2 in IgG1 format, when incubated with human PBMCs for 24 h.
FuncS
Figure 5 The antibody A2 induces ADCC in vivo.
Inhibition of lung metastases formation upon treatment with the antibody A2 in the IgG2a format in mice injected intravenously with C51A33.A5.
Specifications
- Host Species
- Human
- Derivation
- Phage Display Library
- Type
- Human IgG
- Specificity
- Human A33 transmembrane glycoprotein
- Species Reactivity
- Human
- Clone
- A2
- Applications
- ELISA, WB, FC, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- GPA33; glycoprotein A33; A33
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2119z-F(E) | Recombinant Anti-Human GPA33 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| HPAB-1717-FY-S(P) | Human Anti-GPA33 Recombinant Antibody (clone 263A17); Fab Fragment | ELISA | Human Fab |
| HPAB-1718-FY-S(P) | Human Anti-GPA33 Recombinant Antibody (clone 125M10AA); Fab Fragment | ELISA | Human Fab |
| HPAB-0521-YJ-F(E) | Human Anti-GPA33 Recombinant Antibody; Fab Fragment (HPAB-0521-YJ-F(E)) | WB, IP, FC, ELISA, FuncS | Chimeric (Mouse/Human) Fab |
| HPAB-0522-YJ-F(E) | Human Anti-GPA33 Recombinant Antibody; Fab Fragment (HPAB-0522-YJ-F(E)) | WB, IP, FC, ELISA, FuncS | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
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