Rabbit Anti-GYS1 Recombinant Antibody (clone 1D1) (CAT#: ZG-0700J)

Name: Rabbit Anti-GYS1 Recombinant Antibody (clone 1D1)
SKU: ZG-0700J
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes GYS1.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-GYS1 Antibody (ZG-0700J) in WB

Figure 1 Rabbit Anti-GYS1 Antibody (ZG-0700J) in WB

Western Blot
Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-GYS1 antibody at 1.12μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 KDa
Observed band size: 85 KDa

Figure 2 Rabbit Anti-GYS1 Antibody (ZG-0700J) in IF

Figure 2 Rabbit Anti-GYS1 Antibody (ZG-0700J) in IF

Immunofluorescence staining of HepG2 cells with this product at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41598/if_selected.jpg

Normal Tissue

Figure 2 IHC staining of human skeletal muscle
(Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/41598/91527_B_1_6_selected.jpg

Figure 3 Skeletal muscle.
(High expression in skeletal muscle)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/ihc_selected.jpg

Figure 4 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_B_4_6.jpg

Figure 5 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_8_8.jpg

Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_7_3.jpg

Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_9_5.jpg

Figure 8 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7793/18859_A_6_6.jpg

RNA Expression

Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104812-GYS1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human Phospho-GYS1 (S641)

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human GYS1

Species Reactivity
Human

Clone
1D1

Applications
ELISA, WB, IF

Conjugate
Uconjugated

Related Disease
Muscle glycogen storage disease

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
WB:1:500-1:5000, IF:1:20-1:200

Target

Alternative Names
GSY; GYS; glycogen [starch] synthase, muscle; glycogen synthase 1 (muscle)

Gene ID
2997

UniProt ID
P13807

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
MOB-0771MZ	Recombinant Mouse Anti-Human GYS1 Antibody (clone 4B8)	ELISA, ICC/IF, WB	Mouse antibody
MRO-0680-CN	Rabbit Anti-GYS1 Recombinant Antibody (clone CBACN-248)	WB, IF, IHC, IP, FC	Rabbit IgG
VS3-QX491	Mouse Anti-GYS1 Recombinant Antibody (clone 2H1-C11-B9)	WB, IHC, ICC, FC, ELISA	Mouse IgG1
VS3-CJ641	Rabbit Anti-GYS1 (phospho S641) Recombinant Antibody (VS3-CJ641)	WB, ICC, IHC, IP	Rabbit IgG
VS3-CJ642	Rabbit Anti-GYS1 Recombinant Antibody (VS3-CJ642)	WB, ICC, IF, IHC, IP, FC	Rabbit IgG

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