Mouse Anti-HIV-1 gp160 Recombinant Antibody (clone 1575) (CAT#: MRO-3821CQ)

Figure 1 Recognition of GST-p17-IEEE and GST-p17-IEGE with MAb 1575 (16.2 mg/ml).

Both the immunoblot (lower gel) and the Coomassie bluestained
gel of the purified proteins (upper gel) are shown.

Buratti, E., Tisminetzky, S. G., D'Agaro, P., & Baralle, F. E. (1997). A neutralizing monoclonal antibody previously mapped exclusively on human immunodeficiency virus type 1 gp41 recognizes an epitope in p17 sharing the core sequence IEEE. Journal of virology, 71(3), 2457-2462.

Figure 2 ELISA of the FHV hybrid proteins displaying either the p17 sequence (L2p17) or the gp41 sequence (L2gp41) and of the wild-type FHV protein (wt).

The datum points are the products of single ELISA wells, but similar data were obtained by repeating the experiment three times. OD 492, optical density at 492 nm.

Buratti, E., Tisminetzky, S. G., D'Agaro, P., & Baralle, F. E. (1997). A neutralizing monoclonal antibody previously mapped exclusively on human immunodeficiency virus type 1 gp41 recognizes an epitope in p17 sharing the core sequence IEEE. Journal of virology, 71(3), 2457-2462.

Figure 3 Immunofluorescence visualization of binding of MAbs 1575 to infected H9 cells.

Cells were counterstained with Evans blue, and bound antibody was visualized with an FITC-conjugated second antibody.

Buratti, E., Tisminetzky, S. G., D'Agaro, P., & Baralle, F. E. (1997). A neutralizing monoclonal antibody previously mapped exclusively on human immunodeficiency virus type 1 gp41 recognizes an epitope in p17 sharing the core sequence IEEE. Journal of virology, 71(3), 2457-2462.