Mouse Anti-HIV-1 gp160 Recombinant Antibody (clone T32) (CAT#: MRO-3887CQ)

Recombinant mouse antibody to gp160(592-608). T32 binds to region 597-613: WGCSGKLICTTAVPWNA.

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ELISA

Figure 1 E2-derived peptides bind to the disulfide loop of gp41.

Figure 1 E2-derived peptides bind to the disulfide loop of gp41.

Competitive binding of E2 peptides and different gp41 targeting mAbs to immobilized recombinant gp41MN.

Eissmann, K., Mueller, S., Sticht, H., Jung, S., Zou, P., Jiang, S., ... & Reil, H. (2013). HIV-1 fusion is blocked through binding of GB Virus C E2D peptides to the HIV-1 gp41 disulfide loop. PloS one, 8(1), e54452.

WB

Figure 2 293T cells expressing CD4, CCR5, or CXCR4 were preincubated with E2-derived
peptides, E2340-Fc and Fc protein as well as specific controls that bind to gp120.

Figure 2 293T cells expressing CD4, CCR5, or CXCR4 were preincubated with E2-derived peptides, E2340-Fc and Fc protein as well as specific controls that bind to gp120.

No influence of E2 peptides or intact E2 protein on the binding efficiency between
gp120 and CD4, CCR5, or CXCR4 respectively, was observed after normalization of band intensity with the gel loading control Hsp90.

Eissmann, K., Mueller, S., Sticht, H., Jung, S., Zou, P., Jiang, S., ... & Reil, H. (2013). HIV-1 fusion is blocked through binding of GB Virus C E2D peptides to the HIV-1 gp41 disulfide loop. PloS one, 8(1), e54452.

FC

Figure 3 293T cells expressing NL4-3 envelope (X4env) or empty plasmid as control were incubated with sCD4 and FITC-conjugated E2 peptides, respectively.

Figure 3 293T cells expressing NL4-3 envelope (X4env) or empty plasmid as control were incubated with sCD4 and FITC-conjugated E2 peptides, respectively.

Peptide binding was analyzed by flow cytometry.

Eissmann, K., Mueller, S., Sticht, H., Jung, S., Zou, P., Jiang, S., ... & Reil, H. (2013). HIV-1 fusion is blocked through binding of GB Virus C E2D peptides to the HIV-1 gp41 disulfide loop. PloS one, 8(1), e54452.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Recognizes HIV-1 gp160(592-608)
  • Species Reactivity
  • HIV-1
  • Clone
  • T32
  • Applications
  • ELISA, FC, WB

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 gp160 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot.

Target

  • Alternative Names
  • Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP160; gp160; HIV envelope

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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