Mouse Anti-HLA-DRA Recombinant Antibody (clone 1C11A5)
CAT#: VS7-0425-WR72
This product is a mouse IgG2b recombinant antibody that binds to human HLA-DRA. The antibody 1C11A5 recognizes a specific amino acid domains of human HLA-DRA (AA: 26-254). It can be used for WB, ICC, and FC assays.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_A_8_3.jpg
Normal Tissue
Figure 3 High expression in lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_A_7_8.jpg
Normal Tissue
Figure 4 Tonsil
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53176/125541_A_5_8.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, REH, U-266/70, U-698, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204287-HLA-DRA
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Specifications
- Immunogen
- Recombinant human HLA-DRA (AA: 26-254) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human HLA-DRA
- Species Reactivity
- Human
- Clone
- 1C11A5
- Molecule Class
- HLA Class II
- Applications
- WB, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Ankylosing spondylitis; Rheumatoid arthritis; Multiple sclerosis; Type 1 diabetes; Celiac disease; Lupus
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:100 - 1:500
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- HLA-DRA1
- Gene ID
- 3122
- UniProt ID
- P01903
- Long Name
- Major Histocompatibility Complex, Class Ii, Dr Alpha
- Cellular Localization
- Cell membrane; Cytoplasmic vesicle; Endoplasmic reticulum; Endosome; Lysosome; Membrane; MHC II
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Isopeptide bond; Ubl conjugation
- Protein Refseq
- NP_061984
- Function
- Antigen presentation
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Secondary Antibody
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Reconstitution Buffer
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Recommended Dilution Buffer
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