Rabbit Anti-HMOX1 Recombinant Antibody (VS3-CJ541)
CAT#: VS3-CJ541
This product is a rabbit antibody that recognizes human, and mouse HMOX1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/635/51_B8_2_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_8.jpg
Normal Tissue
Figure 5 High expression in spleen
Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_B_8_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, GAMG, U-138 MG, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100292-HMOX1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse HMOX1
- Species Reactivity
- Human, Mouse
- Applications
- WB, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- HO-1; HSP32; HMOX1D; Bk286b10
- Gene ID
- 3162
- UniProt ID
- P09601
- Sequence Similarities
- Belongs to the heme oxygenase family.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- A soluble form arises by proteolytic removal of the membrane anchor.
- Protein Refseq
- NP_002124.1
- Function
- Heme oxygenase 1:
Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:7703255, PubMed:11121422).
Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).
(Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.
Heme oxygenase 1 soluble form:
Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HMOX1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0877CT | Recombinant Mouse anti-Rat HMOX1 Monoclonal antibody (IP-2-3) | ELISA, WB | |
| MRO-1925-CN | Rabbit Anti-HMOX1 Polyclonal Antibody (MRO-1925-CN) | WB, IF, IHC | Rabbit IgG |
| VS3-WK1594 | Mouse Anti-HMOX1 Recombinant Antibody (clone 3F9) | IHC-P, WB | Mouse IgG |
| VS7-0425-WR75 | Mouse Anti-HMOX1 Recombinant Antibody (clone 2B2A1) | IHC, FC | Mouse IgG1 |
| VS7-0425-WR76 | Mouse Anti-HMOX1 Recombinant Antibody (clone 2D5E9) | IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-912CQ | Mouse Anti-HMOX1 Recombinant Antibody (clone GTS-1) | ELISA, IHC-Fr, IHC-P, Inhib, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1639 | Rabbit Anti-HMOX1 Recombinant Antibody (clone DS1639AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY257 | Mouse Anti-NHP HMOX1 Recombinant Antibody (clone 1F12-A6) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1036 | Anti-HMOX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3218 | Anti-Mouse HMOX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3217 | Anti-Human HMOX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3219 | Anti-Bovine HMOX1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC539 | CytoStream™ Rabbit Anti-HMOX1 Recombinant Antibody (VS13-YC539) | WB, IHC-P, IP, FC | Rabbit IgG |
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