Recombinant Rabbit Anti-HMOX1 Antibody (clone R02-6C1)
CAT#: VS3-FY687
This product is a recombinant rabbit antibody that recognizes HMOX1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-6C1 is specific for human, mouse, rat HMOX1. The antigen is recombinant protein of heme oxygenase 1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/635/51_B8_2_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_A_7_8.jpg
Normal Tissue
Figure 5 High expression in spleen
Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17444/39144_B_8_4.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BEWO, GAMG, U-138 MG, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100292-HMOX1
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WB
Figure 1. Recombinant Rabbit Anti-HMOX1 Antibody (clone R02-6C1) in WB.
Western Blot analysis of heme oxygenase 1 in mouse spleen lysates using a heme oxygenase 1 antibody.
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Specifications
- Immunogen
- Recombinant protein of heme oxygenase 1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat HMOX1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6C1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 33 kDa; Observed MW: 33 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Heme Oxygenase 1; Heme Oxygenase (Decycling) 1; HO-1; Heat Shock Protein, 32-KD; EC 1.14.14.18; EC 1.14.99.3
- Gene ID
- 3162
- UniProt ID
- P09601
- Sequence Similarities
- Belongs to the heme oxygenase family.
- Cellular Localization
- Endoplasmic reticulum, Membrane
- Post Translation Modifications
- A soluble form arises by proteolytic removal of the membrane anchor.
- Protein Refseq
- NP_002124.1
- Function
- Heme oxygenase 1:
Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:7703255, PubMed:11121422).
Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).
(Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.
Heme oxygenase 1 soluble form:
Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0877CT | Recombinant Mouse anti-Rat HMOX1 Monoclonal antibody (IP-2-3) | ELISA, WB | |
| MRO-0728-CN | Rabbit Anti-HMOX1 Recombinant Antibody (clone CBACN-265) | WB, IHC, IP, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-912CQ | Mouse Anti-HMOX1 Recombinant Antibody (clone GTS-1) | ELISA, IHC-Fr, IHC-P, Inhib, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1639 | Rabbit Anti-HMOX1 Recombinant Antibody (clone DS1639AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0841 | PE-A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0842 | APC-A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0843 | FITC-A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0844 | A*02:01/Human HMOX1 (QLFEELQEL) MHC Tetramer | FCM, IHC | |
| MHC-CN0845 | PE-A*03:01/Human HMOX1 (QVLKKIAQK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY257 | Mouse Anti-NHP HMOX1 Recombinant Antibody (clone 1F12-A6) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1036 | Anti-HMOX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3218 | Anti-Mouse HMOX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3217 | Anti-Human HMOX1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3219 | Anti-Bovine HMOX1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS13-YC539 | CytoStream™ Rabbit Anti-HMOX1 Recombinant Antibody (VS13-YC539) | WB, IHC-P, IP, FC | Rabbit IgG |
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