Mouse Anti-HNF4A Recombinant Antibody (VS3-CJ57)
CAT#: VS3-CJ57
This product is a mouse antibody that recognizes HNF4A.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/if_selected.jpg
Normal Tissue
Figure 2 Duodenum
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/13034_A_9_2_val_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_3_1.jpg
Normal Tissue
Figure 4 Colon
Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_8_4.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101076-HNF4A
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Specifications
- Immunogen
- Recombinant fragment of human HNF4A (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HNF4A
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
- Gene ID
- 3172
- UniProt ID
- P41235
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR2 subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.
- Protein Refseq
- NP_000448.3; NP_001025174.1; NP_001025175.1
- Function
- Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922).
Activates the transcription of CYP2C38 (By similarity).
Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698).
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Type I Diabetes Mellitus
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "HNF4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2466z | Mouse Anti-HNF4A Recombinant Antibody (clone 35C6) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| MOB-2375MZ | Recombinant Mouse Anti-Human HNF4A Antibody | ICC, IF, WB | Mouse antibody |
| MRO-0757-CN | Rabbit Anti-HNF4A Recombinant Antibody (clone CBACN-280) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ937 | Rabbit Anti-HNF4A Recombinant Antibody (VS3-CJ937) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY690 | Recombinant Mouse Anti-HNF4A Antibody (clone 8D12E3) | ELISA, IHC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1642 | Rabbit Anti-HNF4A Recombinant Antibody (clone DS1642AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3225 | Anti-HNF4A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3226 | Anti-Mouse HNF4A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3227 | Anti-Rat HNF4A Immunohistochemistry Kit | IHC |
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