Rabbit Anti-HNF4A Recombinant Antibody (VS3-CJ937) (CAT#: VS3-CJ937)
This product is a rabbit antibody that recognizes human HNF4A.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/if_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/if_selected.jpg
Figure 2 Duodenum
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/13034_A_9_2_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4712/13034_A_9_2_val_selected.jpg
Figure 3 Stomach
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_3_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_3_1.jpg
Figure 4 Colon
(Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_9_3.jpg
(Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_8_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19417/51797_A_8_4.jpg
Figure 6 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101076-HNF4A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101076-HNF4A
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HNF4A
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha
- Gene ID
- 3172
- UniProt ID
- P41235
- Sequence Similarities
- Belongs to the nuclear hormone receptor family. NR2 subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.
Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
- Protein Refseq
- NP_000448.3; NP_001025174.1; NP_001025175.1
- Function
- Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922).
Activates the transcription of CYP2C38 (By similarity).
Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698).
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
cAMP Signaling Pathway
Type I Diabetes Mellitus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2466z | Mouse Anti-HNF4A Recombinant Antibody (clone 35C6) | WB, ELISA, IF, IHC, IP | Mouse IgG1, κ |
| MRO-0757-CN | Rabbit Anti-HNF4A Recombinant Antibody (clone CBACN-280) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ57 | Mouse Anti-HNF4A Recombinant Antibody (VS3-CJ57) | WB, ELISA | Mouse IgG |
| VS3-FY690 | Recombinant Mouse Anti-HNF4A Antibody (clone 8D12E3) | ELISA, IHC, FC | Mouse IgG2b |
| VS13-YC540 | Rabbit Anti-HNF4A Recombinant Antibody (VS13-YC540) | IHC-P | Rabbit IgG |
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