Rabbit Anti-HNRNPC Recombinant Antibody (VS3-CJ476)
CAT#: VS3-CJ476
This product is a rabbit antibody that recognizes human, mouse, rat, and zebrafish heterogeneous nuclear ribonucleoproteins C (C1/C2).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5223/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75755/1894_G2_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5223/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75755/159385_A_5_8_selected.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5223/13358_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5223/13358_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51075/124984_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51075/124984_A_7_8.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51075/124984_A_9_3.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000092199-HNRNPC
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Zebrafish heterogeneous nuclear ribonucleoproteins C (C1/C2)
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- C1; C2; HNRNP; HNRPC; SNRPC
- Gene ID
- 3183
- UniProt ID
- P07910
- Sequence Similarities
- Belongs to the RRM HNRPC family. RALY subfamily.
- Cellular Localization
- Nucleus, Spliceosome
- Post Translation Modifications
- Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
- Protein Refseq
- NP_001070910.1; NP_001070911.1; NP_004491.2
- Function
- Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621).
Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621).
May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm6A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HNRNPC"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0611CT | Recombinant Mouse anti-Human HNRNPC Monoclonal antibody (5G5) | ELISA, ICC, IF, IHC-P, IP, WB | |
| MRO-0760-CN | Rabbit Anti-HNRNPC Recombinant Antibody (clone CBACN-281) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0741J | Rabbit Anti-HNRNPC Recombinant Antibody (clone 9G1) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
| VS1-YJ99 | Mouse Anti-HNRNPC Recombinant Antibody (clone 4F4) | ELISA, IF, IHC-P, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1580 | PE-B*41:03/Human HNRNPC (AEMYGSVTEHPSPSPL) MHC Tetramer | FCM | |
| MHC-LC1581 | APC-B*41:03/Human HNRNPC (AEMYGSVTEHPSPSPL) MHC Tetramer | FCM | |
| MHC-LC1582 | BV421-B*41:03/Human HNRNPC (AEMYGSVTEHPSPSPL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1646 | Rabbit Anti-HNRNPC Recombinant Antibody (clone DS1646AB) | WB, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1112 | AbPlus™ Anti-HNRNPC Magnetic Beads (VS-0724-YC1112) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1043 | Anti-HNRNPC Immunohistochemistry Kit | IHC | |
| VS-0525-XY3235 | Anti-Mouse HNRNPC Immunohistochemistry Kit | IHC | |
| VS-0525-XY3234 | Anti-Human HNRNPC Immunohistochemistry Kit | IHC |
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