Mouse Anti-HPV16 E7 Recombinant Antibody (clone 69E2) (CAT#: FAMAB-0191WJ)
Clone 69E2 is a mouse antibody target three exposed peptides of the HPV16 E7(49-66), HPV16 E7(73-85), and HPV16 E7(91-97). This antibody can be used in applications, such as ICC, IF, IHC, ELISA, WB, Neutralization.
Specific Inquiry
ELISA
Figure 1 Indirect ELISA assay for the purified mAb titer.
The red dotted line presents S/N ratio = 2.1.
Hu, R., Dong, Z., Zhang, K., Pan, G., Li, C., & Cui, H. (2020). Preparation, Characterization and Diagnostic Valuation of Two Novel Anti-HPV16 E7 Oncoprotein Monoclonal Antibodies. Viruses, 12(3), 333.
ICC
Figure 2 Effectiveness and specificity of the anti-HPV16 E7 protein mAbs identified by using immunocytochemistry.
The 79A11 and 69E2 antibodies at the three dosages of 1.95, 6.25, and 50 µg/mL, respectively, were used to react with the HPV16-type cervical cancer cell line CaSki and the HPV18-type cervical cancer cell line HeLa in immunocytochemical reactions.
Hu, R., Dong, Z., Zhang, K., Pan, G., Li, C., & Cui, H. (2020). Preparation, Characterization and Diagnostic Valuation of Two Novel Anti-HPV16 E7 Oncoprotein Monoclonal Antibodies. Viruses, 12(3), 333.
IF
Figure 3 Effectiveness and specificity of the anti-HPV16 E7 protein mAbs identified by using immunofluorescence.
The 79A11 and 69E2 mAbs, respectively, reacted with the CaSki and HeLa cervical cancer cell lines at a dose of 10 µg/mL in IF experiments.
Hu, R., Dong, Z., Zhang, K., Pan, G., Li, C., & Cui, H. (2020). Preparation, Characterization and Diagnostic Valuation of Two Novel Anti-HPV16 E7 Oncoprotein Monoclonal Antibodies. Viruses, 12(3), 333.
IHC
Figure 4 Effectiveness and specificity of the anti-HPV16 E7 protein mAbs identified by using immunohistochemistry.
The 79A11 and 69E2 mAbs reacted with the paraffin sections of HPV16 cervical squamous cell carcinoma and HPV18 cervical adenocarcinoma tissues at 8.95 and 50 µg/mL, respectively, in IHC assays.
Hu, R., Dong, Z., Zhang, K., Pan, G., Li, C., & Cui, H. (2020). Preparation, Characterization and Diagnostic Valuation of Two Novel Anti-HPV16 E7 Oncoprotein Monoclonal Antibodies. Viruses, 12(3), 333.
WB
Figure 5 Effectiveness and specificity of the anti-HPV16 E7 protein mAbs identified by using Western Blot.
The 79A11 and 69E2 mAbs reacted with CaSki cell protein extract at 10 µg/mL, respectively, in Western Blot experiments.
Hu, R., Dong, Z., Zhang, K., Pan, G., Li, C., & Cui, H. (2020). Preparation, Characterization and Diagnostic Valuation of Two Novel Anti-HPV16 E7 Oncoprotein Monoclonal Antibodies. Viruses, 12(3), 333.
Specifications
- Immunogen
- HPV16 E7-HIS fusion oncoprotein
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG2a
- Specificity
- HPV16 E7
- Species Reactivity
- HPV16
- Clone
- 69E2
- Applications
- ICC, IF, IHC, ELISA, WB, Neut
- Related Disease
- HPV16 infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B047(A) | Recombinant Anti-HPV16 E7 Intrabody [(D-Arg)9] | ICC, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B047(G) | Recombinant Anti-HPV16 E7 Intrabody [+36 GFP] | ELISA, WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B047(T) | Recombinant Anti-HPV16 E7 Intrabody [Tat] | IF, FC, WB, FuncS | scFv-Tat |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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