Rabbit Anti-HSPA5 Recombinant Antibody (clone 4H12) (CAT#: ZG-0749J)
This product is a rabbit antibody that recognizes HSPA5.
Specific Inquiry
IHC
Figure 1 Rabbit Anti-HSPA5 Antibody (ZG-0749J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human GRP78 BiP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSPA5
- Species Reactivity
- Human
- Clone
- 4H12
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
- Alternative Names
- BIP; MIF2; GRP78; HEL-S-89n; endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; HSP70 family protein 5; binding-immunoglobulin protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose-regulated protein, 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 70 family protein 5; heat shock protein family A member 5; immunoglobulin heavy chain-binding protein
- Gene ID
- 3309
- UniProt ID
- P11021
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1425z | Mouse Anti-HSPA5 Recombinant Antibody (clone 23C12) | WB, ICC, IF | Mouse IgG2b |
| MRO-0788-CN | Mouse Anti-HSPA5 Recombinant Antibody (clone A5-C9-G6) | WB, IF, IHC, FC | Mouse IgG |
| MRO-1910-CN | Rabbit Anti-HSPA5 Polyclonal Antibody (MRO-1910-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1911-CN | Rabbit Anti-HSPA5 Polyclonal Antibody (MRO-1911-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1912-CN | Rabbit Anti-HSPA5 Polyclonal Antibody (MRO-1912-CN) | WB, IF, IHC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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