Rabbit Anti-HSPA5 Recombinant Antibody (clone 4H12)
CAT#: ZG-0749J
This product is a rabbit antibody that recognizes HSPA5.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-HSPA5 Antibody (ZG-0749J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
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Specifications
- Immunogen
- A synthesized peptide derived from human GRP78 BiP
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HSPA5
- Species Reactivity
- Human
- Clone
- 4H12
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
- Alternative Names
- BIP; MIF2; GRP78; HEL-S-89n; endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; HSP70 family protein 5; binding-immunoglobulin protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose-regulated protein, 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 70 family protein 5; heat shock protein family A member 5; immunoglobulin heavy chain-binding protein
- Gene ID
- 3309
- UniProt ID
- P11021
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| TAB-009CT | Mouse Anti-HSPA5 Recombinant Antibody (TAB-009CT) | ELISA, Inhib, FC | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
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| MOR-1695 | Rabbit Anti-HSPA5 Recombinant Antibody (clone DS1695AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| NS-035CN | Mouse Anti-HSPA5 Recombinant Antibody (NS-035CN) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-035CN-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (NS-035CN-F(E)) | FC | Mouse Fab |
| NS-035CN-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (NS-035CN-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-S(P) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse scFv |
| HPAB-0369-YJ-S(P) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-F(E) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse Fab |
| HPAB-0369-YJ-F(E) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY234 | Mouse Anti-NHP HSPA5 Recombinant Antibody (clone 6H4-2G7) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY122 | Human Anti-HSPA5 (clone Sam-6) scFv-Fc Chimera | WB, FC, IF, Inhib, ELISA, IHC | Human IgG1, scFv-Fc |
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|---|---|---|---|
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