Mouse Anti-HSPA5 Recombinant Antibody (VS3-CJ582)
CAT#: VS3-CJ582
This product is a mouse antibody that recognizes human, and rat endogenous levels of GRP78 protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_A_7_8.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/38845/85495_B_3_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000044574-HSPA5
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence of human GRP78
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Rat endogenous levels of GRP78 protein
- Species Reactivity
- Human, Rat
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.
- Preservative
- 0.01% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:2000), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- BIP; GRP78; HEL-S-89n
- Gene ID
- 3309
- UniProt ID
- P11021
- Sequence Similarities
- Belongs to the heat shock protein 70 family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum
- Post Translation Modifications
- AMPylated by FICD (PubMed:25601083).
In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins (By similarity).
In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity (By similarity).
- Protein Refseq
- NP_005338.1
- Function
- Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:2294010, PubMed:23769672, PubMed:23990668, PubMed:28332555).
Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity).
Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed:1550958, PubMed:19538957).
In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity).
Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).
Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (PubMed:26045166).
(Microbial infection) Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus (PubMed:33432092, PubMed:15098107, PubMed:28053106).
Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells (PubMed:15098107).
(Microbial infection) Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed:24355926, PubMed:20484814, PubMed:32487760).
Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression (PubMed:32487760).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
| TAB-009CT | Mouse Anti-HSPA5 Recombinant Antibody (TAB-009CT) | ELISA, Inhib, FC | Mouse IgG1 |
| TAB-009CT-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (TAB-009CT-S(P)) | ELISA, Inhib, FC | Mouse scFv |
| TAB-009CT-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (TAB-009CT-F(E)) | ELISA, Inhib, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
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| MOR-1695 | Rabbit Anti-HSPA5 Recombinant Antibody (clone DS1695AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| NS-035CN | Mouse Anti-HSPA5 Recombinant Antibody (NS-035CN) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
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| NS-035CN-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (NS-035CN-F(E)) | FC | Mouse Fab |
| NS-035CN-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (NS-035CN-S(P)) | FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-S(P) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse scFv |
| HPAB-0369-YJ-S(P) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); scFv Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0368-YJ-F(E) | Mouse Anti-HSPA5 Recombinant Antibody (clone MAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Mouse Fab |
| HPAB-0369-YJ-F(E) | Human Anti-HSPA5 Recombinant Antibody (clone huMAb159); Fab Fragment | WB, FC, IHC, ELISA, Inhib, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX555 | Mouse Anti-HSPA5 Recombinant Antibody (clone 6H7-4B1-9H4) | WB, IHC | Mouse IgG1 |
| VS3-QX556 | Mouse Anti-HSPA5 Recombinant Antibody (clone 9C7-8D10-7A5) | WB, IHC | Mouse IgG1 |
| VS3-QX557 | Mouse Anti-HSPA5 Recombinant Antibody (clone 4F11-D5-C10) | WB, IF, IP | Mouse IgG2b |
| VS3-QX558 | Mouse Anti-HSPA5 Recombinant Antibody (clone 9E4-2A7-H6) | WB, ICC, IHC | Mouse IgG1 |
| VS3-QX560 | Mouse Anti-HSPA5 Recombinant Antibody (clone 3B4-H11-C9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-1024-XY234 | Mouse Anti-NHP HSPA5 Recombinant Antibody (clone 6H4-2G7) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY122 | Human Anti-HSPA5 (clone Sam-6) scFv-Fc Chimera | WB, FC, IF, Inhib, ELISA, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3325 | Anti-HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3326 | Anti-Mouse HSPA5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3327 | Anti-Rat HSPA5 Immunohistochemistry Kit | IHC |
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