Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) (CAT#: ZG-0333F)
This product is a mouse antibody that recognizes the epitope C-terminus of the HSPA5.
Specific Inquiry
WB
Figure 1 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in WB.
Western blot detection of BiP/GRP78 (C-terminal) in Hela, C6, Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminal) mouse mAb (1:1000 dilution). Predicted band size: 72KDa. Observed band size: 78KDa.
IF
Figure 2 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in IF.
Immunocytochemical staining was performed on HeLa cells fixed with 4% paraformaldehyde, and anti-BiP/GRP78 (C-terminal) mouse mAb (diluted 1:50) was used.
IHC
Figure 3 Mouse Anti-HSPA5 Recombinant Antibody (ZG-0333F) in IHC.
Immunohistochemical analysis of paraffin-embedded colorectal cancer was performed using BiP/GRP78 (C-terminal) mouse mAb (1/100 dilution). Antigen retrieval is performed by pressure cooking in citrate buffer (pH 6.0).
Specifications
- Immunogen
- Recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPA5
- Species Reactivity
- Human, Rat
- Applications
- Western Blot, Immunocytochemistry
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000
Immunocytochemistry: 1:50
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 5; Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa); Endoplasmic Reticulum Lumenal Ca(2+)-Binding Protein Grp78; Immunoglobulin Heavy Chain-Binding Protein; Glucose-Regulated Protein, 78kDa; GRP78; BIP; Heat Shock 70kD Protein 5 (Glucose-Regulated Protein, 78kD); 78 kDa glucose regulated protein; 78 kDa glucose-regulated reticulum lumenal Ca(2)-binding protein grp78; Endoplasmic reticulum lumenal Ca2 binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP-78; GRP78; GRP78_HUMAN; Heat shock 70 kDa protein 5; Heat Shock 70kDa Protein 5; Hsce70; HSPA 5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; Immunoglobulin heavy chain-binding protein; mBiP; MIF2; Sez7.
- Gene ID
- 3309
- UniProt ID
- P11021
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009CT | Mouse Anti-HSPA5 Recombinant Antibody (TAB-009CT) | ELISA, Inhib, FC | Mouse IgG1 |
| TAB-009CT-S(P) | Mouse Anti-HSPA5 Recombinant Antibody; scFv Fragment (TAB-009CT-S(P)) | ELISA, Inhib, FC | Mouse scFv |
| TAB-009CT-F(E) | Mouse Anti-HSPA5 Recombinant Antibody; Fab Fragment (TAB-009CT-F(E)) | ELISA, Inhib, FC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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