Mouse anti-HSPA8 antibody (clone 2G8F6) displayed high specificity against the HSPA8 protein of Human, Rat and Mouse.
Figure 1 Mouse Anti-HSPA8 Antibody (ZG-0245U) in WB
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue. All lanes HSPA8 antibody at 1: 2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 10s
Figure 2 Mouse Anti-HSPA8 Antibody (ZG-0245U) in IHC
IHC image of ZG-0245U diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
Figure 3 Mouse Anti-HSPA8 Antibody (ZG-0245U) in FC
Overlay histogram showing MCF-7 cells stained with ZG-0245U (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1:200 for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b used under the same conditions. Acquisition of>10,000 events was performed.
Figure 4 Mouse Anti-HSPA8 Antibody (ZG-0245U) in IP
Immunoprecipitating HSPA8 in Hela whole cell lysate. Lane 1: Mouse control IgG2b in Hela whole cell lysate
Lane 2: ZG-0245U (1.5µl) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
For western blotting, the blot was detected with ZG-0245U at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1697 | Rabbit Anti-HSPA8 Recombinant Antibody (clone DS1697AB) | WB, FC, IP, IHC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
ZG-0246U | Mouse Anti-HSPA8 Recombinant Antibody (clone 6E4D2) | ELISA, WB, IHC, IF, FC | Mouse IgG1 |
VS3-CJ583 | Mouse Anti-HSPA8 Recombinant Antibody (VS3-CJ583) | WB, IHC | Mouse IgG |
VS3-CJ584 | Rabbit Anti-HSPA8 Recombinant Antibody (VS3-CJ584) | WB, ICC, IF, IHC, IP | Rabbit IgG |
VS4-WK233 | Mouse Anti-HSPA8 Recombinant Antibody (VS4-WK233) | ELISA, WB | Mouse IgG |
VS3-FY710 | Recombinant Rabbit Anti-HSPA8 Antibody (clone R07-1B7) | WB, IHC-P, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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