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    Mouse Anti-HSPA8 Recombinant Antibody (clone 6C7) (CAT#: VS3-WK1578)

    This antibody is a mouse recombinant antibody against human HSPA8, which functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. HSPA8 also functions as an ATPase in the disassembly of clathrin-coated vesicles during the transport of membrane components through the cell. The clone 6C7 can be used in WB analysis at a dilution of 1/500-2000, ELISA analysis at a dilution of 1/40000, and IHC analysis at a dilution of 1/100-300.

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line CACO-2
    Figure 1 IF staining of human cell line CACO-2
    (Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
    Figure 2 IHC staining of human testis
    Figure 2 IHC staining of human testis
    (Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/ihc_selected.jpg
    Figure 3 IHC staining of human stomach
    Figure 3 IHC staining of human stomach
    (Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/ihc_selected.jpg
    Figure 4 IF staining of human cell line PC-3
    Figure 4 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/987_G4_1_blue_red_green.jpg
    Figure 5 IF staining of human cell line U-2 OS
    Figure 5 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/802_G12_2_blue_red_green.jpg
    Normal Tissue
    Figure 6 Liver
    Figure 6 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_8_4.jpg
    Figure 7 Lymph node
    Figure 7 Lymph node
    (Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_7_8.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8

    Specifications

    • Immunogen
    • Synthesized peptide derived from the Internal region of human HSC 70.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human HSPA8
    • Species Reactivity
    • Human, Rat, Mouse
    • Clone
    • 6C7
    • Applications
    • WB, IHC-P, IF
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein G affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Concentration
    • 1 mg/mL
    • Buffer
    • PBS, 0.5% BSA, 50% Glycerol
    • Preservative
    • 0.02% Sodium Azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/2000
      IHC: 1/100-1/300
      ELISA: 1/40000

    Target

    • Alternative Names
    • Heat Shock Protein Family A (Hsp70) Member 8; Lipopolysaccharide-Associated Protein 1; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; HSPA10; HSC70; HSP73; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Heat Shock Cognate 71 KDa Protein; Epididymis Luminal Protein 33
    • Long Name
    • Heat Shock Protein Family A (Hsp70) Member 8
    • Sequence Similarities
    • Belongs to the heat shock protein 70 family.
    • Cellular Localization
    • Cell membrane, Cytoplasm, Membrane, Nucleus, Spliceosome
    • Post Translation Modifications
    • Acetylated.
      ISGylated.
      Trimethylation at Lys-561 reduces fibrillar SNCA binding.
    • Function
    • Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation.

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MOB-1263z Mouse Anti-HSPA8 Recombinant Antibody (clone 12B9) WB, FC, ICC, IF, IHC, IP Mouse IgM
    MRO-0768-CN Rabbit Anti-HSPA8 Recombinant Antibody (clone CBACN-284) WB, IF, IHC, IP Rabbit IgG
    ZG-0245U Mouse Anti-HSPA8 Recombinant Antibody (clone 2G8F6) ELISA, WB, IHC, FC, IP Mouse IgG2b
    ZG-0246U Mouse Anti-HSPA8 Recombinant Antibody (clone 6E4D2) ELISA, WB, IHC, IF, FC Mouse IgG1
    VS4-WK233 Mouse Anti-HSPA8 Recombinant Antibody (VS4-WK233) ELISA, WB Mouse IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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