Mouse Anti-HSPA8 Recombinant Antibody (clone 6C7)
CAT#: VS3-WK1578
This antibody is a mouse recombinant antibody against human HSPA8, which functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. HSPA8 also functions as an ATPase in the disassembly of clathrin-coated vesicles during the transport of membrane components through the cell. The clone 6C7 can be used in WB analysis at a dilution of 1/500-2000, ELISA analysis at a dilution of 1/40000, and IHC analysis at a dilution of 1/100-300.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/987_G4_1_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52504/802_G12_2_blue_red_green.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_8_4.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2056/5749_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000109971-HSPA8
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Specifications
- Immunogen
- Synthesized peptide derived from the Internal region of human HSC 70.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human HSPA8
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 6C7
- Applications
- WB, IHC-P, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/2000
IHC: 1/100-1/300
ELISA: 1/40000
Target
- Alternative Names
- Heat Shock Protein Family A (Hsp70) Member 8; Lipopolysaccharide-Associated Protein 1; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; HSPA10; HSC70; HSP73; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Heat Shock Cognate 71 KDa Protein; Epididymis Luminal Protein 33
- Gene ID
- 3312
- UniProt ID
- P11142
- Long Name
- Heat Shock Protein Family A (Hsp70) Member 8
- Sequence Similarities
- Belongs to the heat shock protein 70 family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus, Spliceosome
- Post Translation Modifications
- Acetylated.
ISGylated.
Trimethylation at Lys-561 reduces fibrillar SNCA binding.
- Protein Refseq
- NP_006588.1; NP_694881.1
- Function
- Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0939CT | Recombinant Mouse anti-Mouse HSPA8 Monoclonal antibody (24E4) | ICC, IF, IHC-Fr, IP, WB | |
| MRO-1941-CN | Rabbit Anti-HSPA8 Polyclonal Antibody (MRO-1941-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1947-CN | Rabbit Anti-HSPA8 Polyclonal Antibody (MRO-1947-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0245U | Mouse Anti-HSPA8 Recombinant Antibody (clone 2G8F6) | ELISA, WB, IHC, FC, IP | Mouse IgG2b |
| ZG-0246U | Mouse Anti-HSPA8 Recombinant Antibody (clone 6E4D2) | ELISA, WB, IHC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1697 | Rabbit Anti-HSPA8 Recombinant Antibody (clone DS1697AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3328 | Anti-HSPA8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3329 | Anti-Mouse HSPA8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3330 | Anti-Canine HSPA8 Immunohistochemistry Kit | IHC |
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