Mouse Anti-HSPD1 Recombinant Antibody (clone 6C8-G1-B10)

Name: Mouse Anti-HSPD1 Recombinant Antibody (clone 6C8-G1-B10)
SKU: VS3-QX566
Rating: 5 (1 reviews)

CAT#: VS3-QX566

This product is a mouse antibody that recognizes HSPD1. The antibody can be used in applications: WB, IF, IP.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg

Subcellular Location Figure 3 IHC staining of human kidney

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg

Subcellular Location Figure 4 IHC staining of human rectum

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg

Subcellular Location Figure 5 IHC staining of human colon

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg

Subcellular Location Figure 6 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg

Subcellular Location Figure 7 Cerebral cortex

Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg

Normal Tissue Figure 8 Colon

Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg

Normal Tissue Figure 9 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg

Normal Tissue Figure 10 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg

Normal Tissue Figure 11 Kidney

Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg

Normal Tissue Figure 12 Testis

Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg

RNA Expression Figure 13 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1

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Specifications

Immunogen
Purified recombinant human Hsp60 protein fragments expressed in E. coli.

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG2b

Specificity
Hsp60

Species Reactivity
Human, Mouse, Rat

Clone
6C8-G1-B10

Applications
WB, IF, IP

Conjugate
Unconjugated

Product Property

Clonality
Monoclonal

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS (pH 7.4) and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping
Ice packs

Target

Alternative Names
CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13

Gene ID
3329

UniProt ID
P10809

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS3-QX566.pdf

MSDS

MSDS - VS3-QX566.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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