Anti-Human SMAD5 Immunohistochemistry Kit
CAT#: VS-0525-XY6723
This SMAD5 IHC kit ensures high-quality immunostaining with minimal background. It is compatible with both paraffin and frozen tissue sections, offering a flexible tool for studies in signal transduction and developmental processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human uterus, post-menopause
Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
Normal Tissue
Figure 7 Endometrium
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- SMAD5
- Primary Antibody
- Rabbit Anti-SMAD5 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMAD5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3324 | Hi-Affi™ Recombinant Rabbit Anti-SMAD5 Monoclonal Antibody (DS3324AB) | ICC, IF, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1406-CN | Recombinant Rabbit Anti-SMAD5 Monoclonal Antibody (CBACN-514) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2381-CN | Recombinant Rabbit Anti-SMAD5 (phosphorylated Ser463/465) Monoclonal Antibody (CBACN-666) | WB, IF, IHC | Rabbit IgG |
| VS3-CJ97 | Mouse Anti-SMAD5 Recombinant Antibody (VS3-CJ97) | WB, ELISA | Mouse IgG |
| VS3-WK1119 | Rabbit Anti-SMAD5 Recombinant Antibody (VS3-WK1119) | WB, ICC, IHC, FC | Rabbit IgG |
| VS3-WK2102 | Rabbit Anti-SMAD5 (pS463/pS465) Recombinant Antibody (clone R05-2U1) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1079 | CytoStream™ Rabbit Anti-SMAD5 Recombinant Antibody (VS13-YC1079) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2108 | Anti-SMAD5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6724 | Anti-Mouse SMAD5 Immunohistochemistry Kit | IHC |
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