Anti-SMAD5 Immunohistochemistry Kit (CAT#: VS-0325-XY2108)
The SMAD5 IHC Kit offers a complete set of reagents for IHC analysis. It is user - friendly and can be used on both paraffin and frozen sections. The high - specific antibodies in this kit can accurately detect SMAD5, which is beneficial for research on its role in normal and abnormal development, as well as its potential involvement in diseases, especially those related to bone morphogenetic protein (BMP) signaling.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
Figure 2 IHC staining of human uterus, post-menopause
(Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
(Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
Figure 6 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
Figure 7 Endometrium
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- SMAD5
- Primary Antibody
- Rabbit Anti-SMAD5 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
Related Resources
TGF-β Signaling Pathway
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3324 | Hi-Affi™ Recombinant Rabbit Anti-SMAD5 Monoclonal Antibody (DS3324AB) | ICC/IF, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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