CytoStream™ Rabbit Anti-SMAD5 Recombinant Antibody (VS13-YC1079)
CAT#: VS13-YC1079
The CytoStream™ Rabbit Anti-SMAD5 Recombinant Antibody is specific for human and mouse SMAD5. SMAD5 encodes a signaling protein in TGF-beta pathways, managing hematopoietic progenitor cell proliferation. The anti-SMAD5 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human uterus, post-menopause
Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
Normal Tissue
Figure 7 Endometrium
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
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Specifications
- Immunogen
- Recombinant human Smad5, aa 231-282.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse SMAD5
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- SMAD Family Member 5; MADH5; JV5-1; MAD, Mothers Against Decapentaplegic Homolog 5 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 5; SMAD, Mothers Against DPP Homolog 5 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 5; Mothers Against Decapentaplegic Homolog 5; SMAD, Mothers Against DPP Homolog 5;
- Gene ID
- 4090
- UniProt ID
- Q99717
- Long Name
- SMAD Family Member 5
- Sequence Similarities
- Belongs to the dwarfin/SMAD family.
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_001001419.1; NP_001001420.1; NP_005894.3
- Function
- Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SMAD5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1798MZ | Recombinant Mouse Anti-Human SMAD5 Antibody | ICC, IF, WB | Mouse antibody |
| VS3-CJ97 | Mouse Anti-SMAD5 Recombinant Antibody (VS3-CJ97) | WB, ELISA | Mouse IgG |
| VS3-WK1119 | Rabbit Anti-SMAD5 Recombinant Antibody (VS3-WK1119) | WB, ICC, IHC, FC | Rabbit IgG |
| VS3-FY1360 | Recombinant Rabbit Anti-SMAD5 Antibody (clone R07-2A5) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR881 | Mouse Anti-SMAD5 Monoclonal Antibody (VS7-0425-WR881) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3324 | Hi-Affi™ Recombinant Rabbit Anti-SMAD5 Monoclonal Antibody (DS3324AB) | ICC, IF, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2108 | Anti-SMAD5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6724 | Anti-Mouse SMAD5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6723 | Anti-Human SMAD5 Immunohistochemistry Kit | IHC |
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