Anti-IDH1 Antibody Prodrug, Protease Activated (A2E8)
CAT#: VS-1025-YC24
The Anti-IDH1 Antibody Prodrug, Protease Activated (A2E8) is an antibody prodrug comprised of a recombinant mouse antibody targeting human IDH1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to IDH1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to IDH1 effectively. IDH1 (Isocitrate Dehydrogenase 1) is a metabolic enzyme that becomes an oncogene through specific point mutations (most commonly R132H) in lower-grade gliomas and acute myeloid leukemia. This mutation produces an onco-metabolite, 2-hydroxyglutarate (2-HG), which disrupts epigenetic regulation. Inhibitors targeting the mutant IDH1 are a novel therapeutic strategy.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62556/1538_D1_6_selected.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35248/142542_A_7_4.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35248/142542_A_6_6.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35248/142542_A_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Hep G2, RT4)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138413-IDH1
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Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human IDH1
- Target
- IDH1
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- A2E8
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
- Alternative Names
- IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
- Gene ID
- 3417
- UniProt ID
- O75874
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Datasheet
MSDS
COA
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See other products for "Clone A2E8"
- CAT
- Product Name
See other products for "IDH1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1615z | Mouse Anti-IDH1 Recombinant Antibody (clone 27C2) | WB, FC, IF, IHC | Mouse IgG1, κ |
| HPAB-0136-YC | Mouse Anti-IDH1 Recombinant Antibody (clone A2E8) | ELISA, WB, DB | Mouse IgG |
| MRO-0876-CN | Mouse Anti-IDH1 Recombinant Antibody (clone A11-B4) | WB, IHC | Mouse IgG1 |
| VS3-FY721 | Recombinant Rabbit Anti-IDH1 Antibody (clone R05-2G5) | WB, IHC-P, IP | Rabbit IgG |
| VS7-0425-WR112 | Mouse Anti-IDH1 Recombinant Antibody (clone 4A4A8) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1733 | Rabbit Anti-IDH1 Recombinant Antibody (clone DS1733AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0136-YC-S(P) | Mouse Anti-IDH1 Recombinant Antibody (clone A2E8); scFv Fragment | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0136-YC-F(E) | Mouse Anti-IDH1 Recombinant Antibody (clone A2E8); Fab Fragment | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1486 | AbPlus™ Anti-IDH1 Magnetic Beads (VS-0724-YC1486) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY268 | Mouse Anti-NHP IDH1 Recombinant Antibody (clone OTI24A2) | WB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1087 | Anti-IDH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3383 | Anti-Mouse IDH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3382 | Anti-Human IDH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3384 | Anti-Rat IDH1 Immunohistochemistry Kit | IHC |
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