Mouse Anti-IDO1 Recombinant Antibody (clone 19C5)
CAT#: VS11-QX79
This product is a recombinant mouse antibody that recognizes IDO1. This antibody can be used for the following applications: ELISA, IHC. Indoleamine 2,3-dioxygenase 1 (IDO1) catalyzes the initial rate-limiting step in the degradation of the essential amino acid tryptophan along the kynurenine pathway.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm, cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23072/1830_D10_66_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to nucleoplasm, cytosol & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23149/1830_C10_63_selected.jpg
Normal Tissue
Figure 3 Placenta
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23149/51465_A_1_7_val_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23072/51461_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23149/51465_A_9_8.jpg
Normal Tissue
Figure 6 Colon
Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23072/51461_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (EFO-21)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131203-IDO1
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Specifications
- Immunogen
- Recombinant human IDO1 protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human IDO1
- Species Reactivity
- Human
- Clone
- 19C5
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- Indoleamine 2,3-Dioxygenase 1
- Gene ID
- 3620
- UniProt ID
- P14902
- Long Name
- Indoleamine 2,3-dioxygenase 1
- Sequence Similarities
- Belongs to the indoleamine 2,3-dioxygenase family.
- Cellular Localization
- Nucleoplasm, Vesicles, Mitochondria, Cytosol
- Function
- Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IDO1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2702z | Mouse Anti-IDO1 Recombinant Antibody (clone 34C10) | WB, IHC | Mouse IgG2a |
| MOB-2123MZ | Recombinant Mouse Anti-Human IDO1 Antibody | WB | Mouse antibody |
| MRO-1954-CN | Rabbit Anti-IDO1 Polyclonal Antibody (MRO-1954-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0281-YJ | Rabbit Anti-IDO1 Recombinant Antibody (HPAB-0281-YJ) | ELISA, IHC-P, ICC | Rabbit IgG |
| VS3-FY724 | Recombinant Rabbit Anti-IDO1 Antibody (clone R03-5D4) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1735 | Rabbit Anti-IDO1 Recombinant Antibody (clone DS1735AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0281-YJ-S(P) | Rabbit Anti-IDO1 Recombinant Antibody; scFv Fragment (HPAB-0281-YJ-S(P)) | ELISA, IHC-P, ICC | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0281-YJ-F(E) | Rabbit Anti-IDO1 Recombinant Antibody; Fab Fragment (HPAB-0281-YJ-F(E)) | ELISA, IHC-P, ICC | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC394 | AbPlus™ Anti-IDO1 Magnetic Beads (VS-0724-YC394) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1091 | Anti-IDO1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3389 | Anti-Human IDO1 Immunohistochemistry Kit | IHC |
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