Rabbit Anti-IRF3 (pS386) Recombinant Antibody (clone R05-2Q2)
CAT#: VS3-WK2170
This product is a rabbit recombinant antibody that is specific to phosphorylated IRF3. The target of this antibody is phospho-IRF3 (Ser386). The clone R05-2Q2 can be used in WB analysis at a dilution of 1/500-1/1000 and IF analysis at a dilution of 1/50-1/200. The molecular weight of IRF3 (pSer386) observed in WB experiments using this antibody is 47 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4895/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4895/15913_A_1_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32525_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13018/32037_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126456-IRF3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser386 of human IRF3
- Species Reactivity
- Human
- Clone
- R05-2Q2
- Applications
- WB, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
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Related Signaling Pathways
TLR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "IRF3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1855 | Hi-Affi™ Recombinant Rabbit Anti-IRF3 Monoclonal Antibody (DS1855AB) | FC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0771J | Rabbit Anti-IRF3 Recombinant Antibody (clone 4H10) | ELISA, WB | Rabbit IgG |
| VS3-FY763 | Recombinant Mouse Anti-IRF3 Antibody (clone 2A10A8) | ELISA, IHC, ICC, FC | Mouse IgG2b |
| VS3-FY764 | Recombinant Mouse Anti-IRF3 Antibody (clone 5G3E2) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY765 | Recombinant Rabbit Anti-IRF3 Antibody (clone R02-6B0) | WB, IHC-P, IP | Rabbit IgG |
| VS3-WK1587 | Mouse Anti-IRF3 Recombinant Antibody (clone 5H10) | IHC-P, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY285 | Mouse Anti-NHP IRF3 Recombinant Antibody (clone 1522CT766.58.24) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1145 | Anti-IRF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3587 | Anti-Mouse IRF3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3586 | Anti-Human IRF3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC595 | CytoStream™ Rabbit Anti-IRF3 Recombinant Antibody (VS13-YC595) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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